Iyobe S
Laboratory of Drug Resistance in Bacteria, Gunma University School of Medicine.
Nihon Rinsho. 2001 Apr;59(4):701-6.
Metallo-beta-lactamases are the carbapenemases belonging to the class B beta-lactamases on a molecular level. Two types of the metallo-beta-lactamase, IMP-1 and VIM-1, which have been detected mainly from Pseudomonas aeruginosa, respectively, in Japan and in Europe are especially important, because of the broad substrate specificity. These enzymes confer resistance to not only the carbapenems but also almost all beta-lactam antibiotics expect for monobactams. Clinical strains producing IMP-1 type enzymes are easily detectable by the PCR method or by the double-disk method using the enzyme inhibitor of mercapto-compound and the substrate antibiotic such as imipenem or ceftazidime. The gene blaIMP is frequently identified as a cassette inserted in the integron structure on the transmissible plasmids, disseminating among various gram-negative bacteria.
金属β-内酰胺酶在分子水平上属于B类β-内酰胺酶中的碳青霉烯酶。两种类型的金属β-内酰胺酶，IMP-1和VIM-1，在日本主要分别从铜绿假单胞菌中检测到，在欧洲也有发现，它们特别重要，因为具有广泛的底物特异性。这些酶不仅赋予对碳青霉烯类的耐药性，还赋予对除单环β-内酰胺类之外几乎所有β-内酰胺抗生素的耐药性。产生IMP-1型酶的临床菌株可通过PCR方法或使用巯基化合物酶抑制剂和亚胺培南或头孢他啶等底物抗生素的双纸片法轻松检测到。blaIMP基因经常被鉴定为插入可传播质粒上整合子结构中的一个盒式元件，在各种革兰氏阴性菌中传播。
