马来西亚一所大学教学医院中产金属β-内酰胺酶的耐亚胺培南铜绿假单胞菌临床分离株：IMP-7 的检测及 IMP-4、VIM-2 和 VIM-11 的首次鉴定。
Metallo-beta-lactamase-producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11.
机构信息
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
Diagn Microbiol Infect Dis. 2010 Jul;67(3):294-6. doi: 10.1016/j.diagmicrobio.2010.02.010. Epub 2010 May 11.
Ninety (n = 90) imipenem-resistant Pseudomonas aeruginosa (IRPA) clinical isolates collected randomly during 2005 to 2008 from University Malaya Medical Center were assessed for the presence of different variants of metallo-beta-lactamase (MBL) genes. Polymerase chain reaction (PCR) assay detected 32 (n = 32) MBL gene PCR-positive isolates with the presence of bla(IMP) gene in 14 (n = 14) and bla(VIM) in 18 (n = 18) isolates. Four allelic variants, bla(IMP-7) (12 isolates), bla(IMP-4) (2 isolates), bla(VIM-2) (17 isolates), and bla(VIM-11) (1 isolate), of MBL genes were identified. This study is the first report of detection of bla(IMP-4), bla(VIM-2), and bla(VIM-11) MBL genes from IRPA clinical isolates in Malaysia.
本研究从马来西亚大学医学中心随机收集了 2005 年至 2008 年期间的 90 株耐亚胺培南铜绿假单胞菌（IRPA）临床分离株，评估了不同金属β-内酰胺酶（MBL）基因变异体的存在情况。聚合酶链反应（PCR）检测到 32 株 MBL 基因 PCR 阳性分离株，其中 14 株携带 bla(IMP)基因，18 株携带 bla(VIM)基因。鉴定出 4 种等位基因变异体，bla(IMP-7)（12 株）、bla(IMP-4)（2 株）、bla(VIM-2)（17 株）和 bla(VIM-11)（1 株）的 MBL 基因。本研究首次报道了从马来西亚耐亚胺培南铜绿假单胞菌临床分离株中检测到 bla(IMP-4)、bla(VIM-2)和 bla(VIM-11) MBL 基因。
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