使用彗星试验检测硫酸锌对小鼠外周血白细胞的体内遗传毒性作用。

In vivo genotoxic effect of zinc sulfate in mouse peripheral blood leukocytes using Comet assay.

作者信息

Banu B S, Devi K D, Mahboob M, Jamil K

机构信息

Indian Institute of Chemical Technology, Biology and Biotechnology Division, Toxicology Unit, Hyderabad, A.P., India 500 007.

出版信息

Drug Chem Toxicol. 2001 Feb;24(1):63-73. doi: 10.1081/dct-100103086.

DOI:10.1081/dct-100103086

PMID:11307635

Abstract

Single stranded DNA breaks induced by Zinc sulfate in mice has been studied in vivo using Alkaline Single Cell Gel Electrophoresis (Comet assay). Mice were administered orally with doses of 5.70, 8.55, 11.40, 14.25, 17.10 and 19.95 mg/kg body weight of zinc sulfate respectively. The samples of whole blood were collected at 24, 48, 72, 96 hr and first week post-treatment and the assay was carried out to determine single strand DNA breaks as represented by comet tail-lengths. Results indicated a significant DNA damage at all the doses after treatment with zinc sulfate when compared to controls showing a clear dose-dependent response (p < 0.05). A gradual decrease in the tail-lengths from 48 hr post-treatment onwards was observed indicating a time dependent decrease in the DNA damage. The study confirms that zinc sulfate causes significant DNA damage at the doses used as revealed by comet assay.

摘要

利用碱性单细胞凝胶电泳（彗星试验）在体内研究了硫酸锌诱导的小鼠单链DNA断裂情况。分别给小鼠口服5.70、8.55、11.40、14.25、17.10和19.95毫克/千克体重的硫酸锌。在治疗后24、48、72、96小时以及第一周采集全血样本，并进行试验以确定以彗星尾长表示的单链DNA断裂情况。结果表明，与对照组相比，硫酸锌处理后所有剂量均造成了显著的DNA损伤，呈现出明显的剂量依赖性反应（p < 0.05）。观察到从治疗后48小时起尾长逐渐缩短，表明DNA损伤呈时间依赖性减少。该研究证实，彗星试验显示，所用剂量的硫酸锌会造成显著的DNA损伤。