Neff W E, List G R, Byrdwell W C
US Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL 61604, USA.
J Chromatogr A. 2001 Mar 30;912(1):187-90. doi: 10.1016/s0021-9673(01)00544-1.
Reversed-phase HPLC resolution and HPLC-flame ionization detection quantitation of model triacylglycerol positional isomer pairs (important in the study of food formulation lipids) after facile conversion to brominated derivatives is reported. The positional isomers in the triacylglycerol pairs were at least 98% resolved from each other during reversed-phase HPLC. Triacylglycerol quantitation obtained by HPLC-flame ionization detector was checked against standard positional isomer pairs known by mass. The flame ionization detection area percent gave absolute error range of 0.3-1.6% per triacylglycerol.
据报道，在将模型三酰甘油位置异构体对（在食品配方脂质研究中很重要）轻松转化为溴化衍生物后，采用反相高效液相色谱法进行分离，并通过高效液相色谱-火焰离子化检测法定量。在反相高效液相色谱过程中，三酰甘油对中的位置异构体彼此之间的分离度至少为98%。通过高效液相色谱-火焰离子化检测器获得的三酰甘油定量结果与已知质量的标准位置异构体对进行了对照。火焰离子化检测面积百分比给出的每三酰甘油绝对误差范围为0.3 - 1.6%。
