Norman H A, St John J B
J Lipid Res. 1986 Oct;27(10):1104-7.
A method is described for the direct quantitation of phosphatidylcholine molecular species by reverse phase high-performance liquid chromatography employing flame ionization detection. The method is shown to be applicable to plan phosphatidylcholine. The molecular species are separated with a C18 column eluted in an isocratic mode. Detection by a commercially available flame ionization detector overcomes the problems of detecting underivatized naturally occurring lipids using ultraviolet detectors, and allows direct and rapid mass determination of the resolved molecular species. Detection limits for quantitation are defined.
本文描述了一种采用火焰离子化检测的反相高效液相色谱法直接定量磷脂酰胆碱分子种类的方法。该方法适用于植物磷脂酰胆碱。分子种类通过在等度模式下洗脱的C18柱进行分离。使用市售的火焰离子化检测器进行检测克服了使用紫外检测器检测未衍生化天然存在脂质的问题，并允许对分离的分子种类进行直接和快速的质量测定。定义了定量检测限。
