Sepulveda R, Longbottom J L, Pepys J
Clin Allergy. 1979 Jul;9(4):359-71. doi: 10.1111/j.1365-2222.1979.tb02494.x.
ELISA has emerged as a useful alternative to other more costly and complex tests. Polystyrene microhaemagglutination plates have been used as solid phase to absorb Aspergillus fumigatus protein and polysaccharide components for detection of specific antibodies in patients with various forms of pulmonary aspergillosis. IgG and IgE antibodies to the polysaccharide as well as the protein allergens have been found. For the IgE test a double antibody technique has been developed, which is more sensitive than the conventional indirect ELISA.
酶联免疫吸附测定（ELISA）已成为一种有用的方法，可替代其他成本更高且更复杂的检测。聚苯乙烯微量血凝板已被用作固相，以吸附烟曲霉的蛋白质和多糖成分，用于检测各种形式肺曲霉病患者的特异性抗体。已发现针对多糖以及蛋白质过敏原的IgG和IgE抗体。对于IgE检测，已开发出一种双抗体技术，该技术比传统的间接ELISA更灵敏。
