Weitz H J, Ritchie J M, Bailey D A, Horsburgh A M, Killham K, Glover L A
Department Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresthill, UK.
FEMS Microbiol Lett. 2001 Apr 13;197(2):159-65. doi: 10.1111/j.1574-6968.2001.tb10598.x.
A mini-Tn5 transposon was modified to introduce a promoterless luxCDABE cassette from Vibrio fischeri into environmentally relevant bacterial strains in order to develop bioluminescence-based biosensors for toxicity testing. The mini-Tn5 luxCDABE transposon was chromosomally integrated downstream from an active promoter into two Pseudomonas strains (Pseudomonas fluorescens 8866 and Pseudomonas putida F1). Characterisation of the bioluminescent transconjugants demonstrated that the transposon integration was stable and had no effect on growth rate. Both P. fluorescens 8866 Tn5 luxCDABE and P. putida F1 Tn5 luxCDABE were used to assess the toxicity of standard solutions (Cu, Zn and 3,5-DCP) as well as Cu- and 3,5-DCP-spiked groundwater samples. They were successfully used for bioluminescence-based bioassays and the potential value of using different bacterial biosensors for ecotoxicity testing was shown.
对一个微型Tn5转座子进行了改造，以便将来自费氏弧菌的无启动子luxCDABE操纵子引入与环境相关的细菌菌株中，从而开发基于生物发光的生物传感器用于毒性测试。微型Tn5 luxCDABE转座子通过染色体整合，在一个活性启动子的下游整合到两种假单胞菌菌株（荧光假单胞菌8866和恶臭假单胞菌F1）中。对生物发光转接合子的表征表明，转座子整合是稳定的，并且对生长速率没有影响。荧光假单胞菌8866 Tn5 luxCDABE和恶臭假单胞菌F1 Tn5 luxCDABE均用于评估标准溶液（铜、锌和3,5-二氯苯酚）以及添加了铜和3,5-二氯苯酚的地下水样品的毒性。它们成功地用于基于生物发光的生物测定，并显示了使用不同细菌生物传感器进行生态毒性测试的潜在价值。
