Albertson N H, Stretton S, Pongpattanakitshote S, Ostling J, Marshall K C, Goodman A E, Kjelleberg S
Department of General and Marine Microbiology, Lundberg Laboratory, University of Göteborg, Sweden.
FEMS Microbiol Lett. 1996 Jul 1;140(2-3):287-94. doi: 10.1016/0378-1097(96)00196-6.
The previously described pLOFKm transposon delivery plasmid (J.Bacteriol. (1990) 172, 6557-6567) was engineered such that a promotorless lacZ gene was cloned within the transposon cassette, generating the vector pLBT. Using pLBT, stable insertion mutations were generated at high frequencies in Vibrio sp. S141 and Pseudomonas sp. S91, and the interrupted genes could be monitored for their pattern of regulation. Genetic screens isolated mutants defective in a variety of activities. We describe the construction and use of pLBT as a tool for reporter gene mutant analysis in bacteria other than well-characterized laboratory strains.
先前描述的pLOFKm转座子递送质粒（《细菌学杂志》（1990年）172卷，6557 - 6567页）经过改造，使得一个无启动子的lacZ基因被克隆到转座子盒内，从而产生载体pLBT。使用pLBT，在弧菌属S141菌株和假单胞菌属S91菌株中以高频率产生了稳定的插入突变，并且可以监测被中断基因的调控模式。遗传筛选分离出了在多种活性方面存在缺陷的突变体。我们描述了pLBT的构建及其作为一种工具在除了特征明确的实验室菌株之外的细菌中进行报告基因突变分析的用途。
