用于修复兔髁突关节软骨全层缺损的软骨细胞同种异体移植物

Chondrocyte allografts for repair of full-thickness defects in the condylar articular cartilage of rabbits.

作者信息

Yao X, Ma X, Zhang Z

机构信息

Center for Temporomandibular Disorders (TMD), School of Stomatology, Beijing Medical University (BMU), Beijing 100081, P. R. China.

出版信息

Chin J Dent Res. 2000 Nov;3(3):24-30.

PMID:11314531

Abstract

PURPOSE

This study investigated the feasibility of repairing defects of the condylar articular cartilage by chondrocyte allotransplantation.

MATERIAL AND METHODS

A full-thickness defect (2 mm diameter) was made in the condylar articular cartilage of 6-month-old rabbits with a No. 701 dental fissure bur that penetrated both the articular cartilage and the subchondral bone, and entered the marrow cavity. Sixty-four animals were divided into 5 groups. In the cell transplantation group (20 rabbits), the defect was filled with a collagen membrane embedded with chondrocytes of neonate rabbits cultured in vitro for 1 week. In control group 1 (18 rabbits), the defect was left untreated. In control group 2 (18 rabbits), the defect was filled with a collagen membrane without chondrocytes. In sham-operation control group (6 rabbits), the condyle surface was exposed but left unchanged. Two rabbits were added as a normal control group. Mandibular movement was not restricted postoperatively. The macroscopic and microscopic features of the condyles were observed at 1, 2, 4, 8, 12, and 20 weeks following surgery.

RESULTS

The defects in the condylar articular cartilage were repaired with cartilage tissue after cell transplantation.

CONCLUSION

A defect in the condylar articular cartilage can be repaired with articular cartilage-like tissue by allotransplantation of chondrocytes.

摘要

目的

本研究探讨同种异体软骨细胞移植修复髁突关节软骨缺损的可行性。

材料与方法

用701号牙科裂钻在6月龄兔的髁突关节软骨上制造全层缺损（直径2mm），穿透关节软骨和软骨下骨并进入骨髓腔。64只动物分为5组。细胞移植组（20只兔），缺损处填充嵌入体外培养1周的新生兔软骨细胞的胶原膜。对照组1（18只兔），缺损处不做处理。对照组2（18只兔），缺损处填充无软骨细胞的胶原膜。假手术对照组（6只兔），暴露髁突表面但不做改变。另加2只兔作为正常对照组。术后下颌运动不受限制。术后1、2、4、8、12和20周观察髁突的宏观和微观特征。