Płończak M, Czubak J, Kawiak J
Post-Graduate Medical Education Center, Gruca Teaching Hospital, ul.Wczasowa 21B/8, 05-402 Otwock, Poland.
Transplant Proc. 2006 Jan-Feb;38(1):312-3. doi: 10.1016/j.transproceed.2005.12.031.
Autologous osteochondral transplantation is one method that can be used to create hyaline or hyaline-like repair in a defect area. The purpose of the present study was to repair full-thickness articular cartilage defects in 9 rabbit knee joints with autologous cultured chondrocytes.
An articular cartilage defect was created on the patellar groove of the femur. The defect was filled with chondrocytes cultured in vitro and placed into the knee on a polysulphonic membrane. At 8 weeks after the operation, the reparative tissue was analyzed macroscopically and histologically.
At 8 weeks after the operation, the surfaces of the reparative tissue were smooth, and the defects were filled with mature hyaline cartilage in 5 cases. In 2 cases, the reparative hyaline cartilage was immature and there was worse integration of grafted tissue into the adjacent normal cartilage. In 2 cases, the surface of the grafted area was irregular, and the reparative tissue was disintegrated and incompletely differentiated.
The results suggest that transplantation of autologous chondrocytes cultured in vitro and placed into the knee on polysulphonic membrane is effective in repairing an articular cartilage defect.
自体骨软骨移植是一种可用于在缺损区域形成透明软骨或类透明软骨修复的方法。本研究的目的是用自体培养软骨细胞修复9只兔膝关节的全层关节软骨缺损。
在股骨髌沟处制造关节软骨缺损。缺损处填充体外培养的软骨细胞，并置于膝关节的聚砜膜上。术后8周，对修复组织进行宏观和组织学分析。
术后8周，修复组织表面光滑，5例缺损处充满成熟透明软骨。2例中，修复的透明软骨不成熟，移植组织与相邻正常软骨的整合较差。2例中，移植区域表面不规则，修复组织解体且分化不完全。
结果表明，体外培养并置于膝关节聚砜膜上的自体软骨细胞移植对修复关节软骨缺损有效。
