Jin M, Hjortberg M, Hamren J, Sundström P, Nilsson B O
Department of Human Anatomy, University of Uppsala, Sweden.
Ital J Anat Embryol. 1998;103(4 Suppl 1):129-36.
Monoclonal antibodies were raised against zona-free mouse oocytes by intrasplenic immunizations. Antibodies, which detected antigen epitopes in unfertilized but not in fertilized mouse eggs (fertilization-specific antibodies) and which in addition recognized epitopes in unfertilized human oocytes (mouse-human cross-reacting antibodies) were used to visualize the distribution of their corresponding antigens by confocal laser fluoresence scanning microscopy (CLFSM). We found that among 26 antibodies tested, two ones showed a patch pattern. Since a patchy distribution of cell membrane molecules indicates the presence of integral proteins with ability to transduce signals to the cytoplasm, we suggest that the method here applied will select for those oolemma molecules that could be involved in the fusion of sperm cells.
通过脾内免疫接种制备了针对无透明带小鼠卵母细胞的单克隆抗体。这些抗体能够检测未受精小鼠卵中的抗原表位，但不能检测受精小鼠卵中的抗原表位（受精特异性抗体），此外还能识别未受精人卵母细胞中的表位（小鼠 - 人交叉反应抗体），利用共聚焦激光荧光扫描显微镜（CLFSM）来观察其相应抗原的分布。我们发现，在所测试的26种抗体中，有两种呈现出斑块状模式。由于细胞膜分子的斑块状分布表明存在能够将信号转导至细胞质的整合蛋白，我们认为这里应用的方法将筛选出那些可能参与精子细胞融合的卵膜分子。
