Barańska W, Konwiński M, Kujawa M
J Exp Zool. 1975 May;192(2):193-202. doi: 10.1002/jez.1401920209.
The fine structure of the zona pellucida of mouse unfertilized egg cells and of mouse embryos was investigated. In addition to standard fixation for electron microscopy, fixation with an addition of ruthenium red was used and found to be especially valuable for the purpose of studying the zona pellucida. The zona pellucida of unfertilized egg cells and of embryos consisted mainly of fibrillar and granular material. There were two layers: a thicker internal and a denser external layer in the zona pellucida of the unfertilized egg cells. The zona pellucida of embryos had, in addition to these two layers, a coarse-grained intimal layer inside the internal layer and a fine-grained peripheral cortical reaction whereas the peripheral layer can be accumulated and modified during the passage of the embryo through the female genital tract. The influence of developmental events and environmental factors on the fine structure of zona pellucida was discussed.
对小鼠未受精卵细胞和小鼠胚胎透明带的精细结构进行了研究。除了用于电子显微镜检查的标准固定方法外，还使用了添加钌红的固定方法，发现该方法对于研究透明带特别有价值。未受精卵细胞和胚胎的透明带主要由纤维状和颗粒状物质组成。有两层：未受精卵细胞透明带中较厚的内层和较致密的外层。胚胎的透明带除了这两层外，在内层内部还有一层粗颗粒内膜层，以及一层细颗粒外周皮质反应层，而外周层在胚胎通过雌性生殖道的过程中会积累并发生改变。讨论了发育事件和环境因素对透明带精细结构的影响。
