Ohtori S, Takahashi K, Chiba T, Yamagata M, Sameda H, Moriya H
Third Department of Anatomy, School of Medicine, Chiba University, Chiba, Japan.
Spine (Phila Pa 1976). 2001 Apr 15;26(8):946-50. doi: 10.1097/00007632-200104150-00020.
The levels of dorsal root ganglions (DRGs) innervating the dorsal portion of the lumbar intervertebral discs from L1-L2 to L4-L5 were investigated in rats by the retrograde transport method. The pathways and functions of nerve fibers supplying the dorsal portion of the discs were investigated by denervation and immuno-histochemistry.
To investigate the sensory innervation of the dorsal portion of the lumbar intervertebral discs in rats.
The dorsal portion of the L5-L6 disc has been reported to be innervated multisegmentally, and anesthetic blockade of the paravertebral sympathetic trunks and the L2 spinal nerve can relieve discogenic low back pain. However, sensory innervation of the dorsal portion of the lumbar discs at other levels has not been clarified.
A retrograde transport of Fluoro-Gold was used. We studied a nonsympathectomy group (n = 44) and a sympathectomy group (n = 50) in which paravertebral sympathetic trunks were resected from L1 to L5 levels. Using a ventral approach, Fluoro-Gold crystals were inserted into the dorsal portion of the L1-L2, L2-L3, L3-L4, and L4-L5 discs. Seven days after surgery, Fluoro-Gold-labeled neurons were counted in the bilateral dorsal root ganglions from T10 to L6.
Fluoro-Gold-labeled neurons were distributed in dorsal root ganglions from T11 to L5 levels in the nonsympathectomy group. However, in the sympathectomy group the number of labeled neurons was less than that of the nonsympathectomy group in T11, T12, and T13 dorsal root ganglions of the L1-L2 disc group, in T12, T13, and L1 dorsal root ganglions of the L2-L3 disc group, in T12, T13, L1, and L2 dorsal root ganglions of the L3-L4 disc group, and in T12, T13, L1, and L2 dorsal root ganglions of the L4-L5 disc group.
The dorsal portion of the lumbar discs from L1-L2 to L4-L5 is multisegmentally innervated by the T11 through L5 dorsal root ganglions. Sensory fibers from the upper dorsal root ganglions innervate the dorsal portion of the discs via the paravertebral sympathetic trunks, although those from the lower dorsal root ganglions innervate via the sinuvertebral nerves. Furthermore, sensory nerve fibers enter the paravertebral sympathetic trunks through the corresponding ramus communicans and reach the dorsal root ganglions via each ramus communicans at the L2 and/or more cranial levels.
采用逆行运输法在大鼠中研究支配L1-L2至L4-L5腰椎间盘背侧部分的背根神经节（DRG）水平。通过去神经支配和免疫组织化学研究供应椎间盘背侧部分的神经纤维的通路和功能。
研究大鼠腰椎间盘背侧部分的感觉神经支配。
据报道，L5-L6椎间盘的背侧部分由多节段神经支配，椎旁交感干和L2脊神经的麻醉阻滞可缓解盘源性下腰痛。然而，其他节段腰椎间盘背侧部分的感觉神经支配尚未明确。
使用荧光金逆行运输法。我们研究了一个非交感神经切除术组（n = 44）和一个交感神经切除术组（n = 50），其中从L1至L5水平切除椎旁交感干。采用腹侧入路，将荧光金晶体插入L1-L2、L2-L3、L3-L4和L4-L5椎间盘的背侧部分。术后7天，计数T10至L6双侧背根神经节中荧光金标记的神经元。
在非交感神经切除术组中，荧光金标记的神经元分布在T11至L5水平的背根神经节中。然而，在交感神经切除术组中，L1-L2椎间盘组的T11、T12和T13背根神经节、L2-L3椎间盘组的T12、T13和L1背根神经节、L3-L4椎间盘组的T12、T13、L1和L2背根神经节以及L4-L5椎间盘组的T12、T13、L1和L2背根神经节中，标记神经元的数量少于非交感神经切除术组。
L1-L2至L4-L5的腰椎间盘背侧部分由T11至L5背根神经节多节段神经支配。来自上背根神经节的感觉纤维通过椎旁交感干支配椎间盘的背侧部分，而来自下背根神经节的感觉纤维则通过窦椎神经支配。此外，感觉神经纤维通过相应的交通支进入椎旁交感干，并在L2和/或更高的颅侧水平通过每个交通支到达背根神经节。
