通过高效尺寸排阻色谱法测定透析液和超滤液样本中的白蛋白和肌红蛋白。

Determination of albumin and myoglobin in dialysate and ultrafiltrate samples by high-performance size-exclusion chromatography.

作者信息

Liang H, Scott M K, Murry D J, Sowinski K M

机构信息

Purdue University, School of Pharmacy and Pharmacal Sciences, Department of Pharmacy Practice, Wishard Memorial Hospital, Indianapolis, IN 46202, USA.

出版信息

J Chromatogr B Biomed Sci Appl. 2001 Apr 15;754(1):141-51. doi: 10.1016/s0378-4347(00)00600-9.

DOI:10.1016/s0378-4347(00)00600-9

PMID:11318409

Abstract

A high-performance size-exclusion chromatographic method was developed, validated and implemented for simultaneous and quantitative determination of albumin and myoglobin along with inulin, vancomycin and creatinine in dialysate and ultrafiltrate samples from in vitro hemodialysis experiments. The experimental parameters including mobile phase pH, ionic strength, detection wavelength, flow-rate, injection volume were first optimized for the determination of albumin, myoglobin, inulin, vancomycin and creatinine. The peak height ratio and detection limits of the proteins were then comparatively studied at 210, 254 and 280 nm by UV and diode array detection. The method was further validated by evaluating the linearity, precision and accuracy of the proteins. The assay was finally implemented to the simultaneous and quantitative determination of the proteins in dialysate and ultrafiltrate samples.

摘要

开发、验证并实施了一种高效尺寸排阻色谱法，用于同时定量测定体外血液透析实验中透析液和超滤液样品中的白蛋白、肌红蛋白以及菊粉、万古霉素和肌酐。首先对包括流动相pH值、离子强度、检测波长、流速、进样体积在内的实验参数进行优化，以测定白蛋白、肌红蛋白、菊粉、万古霉素和肌酐。然后通过紫外和二极管阵列检测在210、254和280 nm波长下对蛋白质的峰高比和检测限进行了比较研究。通过评估蛋白质的线性、精密度和准确度对该方法进行了进一步验证。该测定法最终用于同时定量测定透析液和超滤液样品中的蛋白质。