透析废液中肌酐的光学测量。

Optical measurement of creatinine in spent dialysate.

作者信息

Tomson Ruth, Fridolin Ivo, Uhlin Fredrik, Holmar Jana, Lauri Kai, Luman Merike

机构信息

Department of Biomedical Engineering, Technomedicum, Tallinn University of Technology, Tallinn, Estonia.

出版信息

Clin Nephrol. 2013 Feb;79(2):107-17. doi: 10.5414/CN107338.

DOI:10.5414/CN107338

PMID:23110769

Abstract

AIM

The aim of the study was to develop an optical method for the estimation of creatinine (Cr) removal during dialysis using UV-absorbance.

MATERIAL AND METHODS

29 hemodialysis patients on chronic 3-times-a-week hemodialysis were studied in 6 separate studies. Double-beam pectrophotometer was used for the determination of UV-absorbance in the collected spent dialysate samples. A single wavelength (SW) and a multi-wavelength (MW) model were developed using stepwise regression utilizing Cr values from the laboratory as the dependent parameter. The reduction ratio (RR) and total removed Cr (TRCr) were estimated.

RESULTS

For blood-Cr RRb (mean ± SD) was 60.9 ± 5.0% (calibration set) and 58.1 ± 6.0% (validation set), for SW UVabsorbance RR_SW was 61.5 ± 5.9% and 57.3 ± 6.0%, and for MW UV-absorbance RR_MW was 65.8 ± 5.8% and 61.7 ± 6.4% respectively. RR_SW and RRb were not statistically different. RR_MW was higher compared to RRb (p < 0.05). TRCr_lab was 13.8 ± 3.8 mmol, TRCr_SW 14.5 ± 2.5 mmol and TRCr_MW 13.8 ± 2.6 mmol, being not statistically different.

CONCLUSION

In summary, creatinine removal during dialysis can be estimated as reduction ratio and total removed creatinine with the UV-absorbance technique.

摘要

目的

本研究的目的是开发一种利用紫外吸光度估算透析过程中肌酐（Cr）清除率的光学方法。

材料与方法

在6项独立研究中对29例每周进行3次慢性血液透析的患者进行了研究。使用双光束分光光度计测定收集的透析废液样本中的紫外吸光度。以实验室测定的Cr值作为因变量，采用逐步回归法建立了单波长（SW）和多波长（MW）模型。估算了清除率（RR）和总清除肌酐量（TRCr）。

结果

血Cr的RRb（均值±标准差）在校准组为60.9±5.0%，在验证组为58.1±6.0%；SW紫外吸光度的RR_SW分别为61.5±5.9%和57.3±6.0%；MW紫外吸光度的RR_MW分别为65.8±5.8%和61.7±6.4%。RR_SW与RRb无统计学差异。RR_MW高于RRb（p<0.05）。实验室测定的TRCr_lab为13.8±3.8 mmol，TRCr_SW为14.5±2.5 mmol，TRCr_MW为13.8±2.6 mmol，无统计学差异。