Sicher Richard C., Bunce James A.
U.S.D.A., Agricultural Research Service, Climate Stress Laboratory, Bldg. 046-A, 10300 Baltimore Avenue, Beltsville Agricultural Research Center - West, Beltsville, MD 20705-2350, USA.
Physiol Plant. 2001 May;112(1):55-61. doi: 10.1034/j.1399-3054.2001.1120108.x.
Single leaf photosynthetic rates and various leaf components of potato (Solanum tuberosum L.) were studied 1-3 days after reciprocally transferring plants between the ambient and elevated growth CO2 treatments. Plants were raised from individual tuber sections in controlled environment chambers at either ambient (36 Pa) or elevated (72 Pa) CO2. One half of the plants in each growth CO2 treatment were transferred to the opposite CO2 treatment 34 days after sowing (DAS). Net photosynthesis (Pn) rates and various leaf components were then measured 34, 35 and 37 DAS at both 36 and 72 Pa CO2. Three-day means of single leaf Pn rates, leaf starch, glucose, initial and total Rubisco activity, Rubisco protein, chlorophyll (a+b), chlorophyll (a/b), alpha-amino N, and nitrate levels differed significantly in the continuous ambient and elevated CO2 treatments. Acclimation of single leaf Pn rates was partially to completely reversed 3 days after elevated CO2-grown plants were shifted to ambient CO2, whereas there was little evidence of photosynthetic acclimation 3 days after ambient CO2-grown plants were shifted to elevated CO2. In a four-way comparison of the 36, 72, 36 to 72 (shifted up) and 72 to 36 (shifted down) Pa CO2 treatments 37 DAS, leaf starch, soluble carbohydrates, Rubisco protein and nitrate were the only photosynthetic factors that differed significantly. Simple and multiple regression analyses suggested that negative changes of Pn in response to growth CO2 treatment were most closely correlated with increased leaf starch levels.
在将马铃薯（Solanum tuberosum L.）植株在环境生长二氧化碳处理和高浓度生长二氧化碳处理之间相互转移1至3天后，对其单叶光合速率和各种叶片成分进行了研究。植株由单个块茎切段在可控环境舱中培养，生长环境的二氧化碳浓度分别为环境浓度（36 Pa）或高浓度（72 Pa）。在播种后34天（DAS），将每种生长二氧化碳处理中的一半植株转移到相反的二氧化碳处理环境中。然后在34、35和37 DAS时，分别在36 Pa和72 Pa二氧化碳浓度下测量净光合速率（Pn）和各种叶片成分。在持续的环境二氧化碳处理和高浓度二氧化碳处理中，单叶Pn速率、叶片淀粉、葡萄糖、初始和总Rubisco活性、Rubisco蛋白、叶绿素（a + b）、叶绿素（a/b）、α-氨基氮和硝酸盐水平的三日均值存在显著差异。高浓度二氧化碳培养的植株转移到环境二氧化碳环境3天后，单叶Pn速率的适应部分至完全逆转，而环境二氧化碳培养的植株转移到高浓度二氧化碳环境3天后，几乎没有光合适应的迹象。在37 DAS时对36、72、36至72（上调）和72至36（下调）Pa二氧化碳处理进行的四向比较中，叶片淀粉、可溶性碳水化合物、Rubisco蛋白和硝酸盐是仅有的显著不同的光合因子。简单和多元回归分析表明，Pn对生长二氧化碳处理的负向变化与叶片淀粉水平的增加最密切相关。
