艰难梭菌的Tn5397、粪肠球菌的Tn916和产气荚膜梭菌的CW459tet(M)元件的比较表明，它们具有相似的接合区域，但插入和切除模块不同。

Comparison of Tn5397 from Clostridium difficile, Tn916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules.

作者信息

Roberts Adam P, Johanesen Priscilla A, Lyras Dena, Mullany Peter, Rood Julian I

机构信息

Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray's Inn Road, London WC1X 8LD, UK1.

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Vic 3800, Australia2.

出版信息

Microbiology (Reading). 2001 May;147(Pt 5):1243-1251. doi: 10.1099/00221287-147-5-1243.

DOI:10.1099/00221287-147-5-1243

PMID:11320127

Abstract

Comparative analysis of the conjugative transposons Tn5397 from Clostridium difficile and Tn916 from Enterococcus faecalis, and the CW459tet(M) element from Clostridium perfringens, has revealed that these tetracycline-resistance elements are closely related. All three elements contain the tet(M) resistance gene and have sequence similarity throughout their central region. However, they have very different integration/excision modules. Instead of the int and xis genes that are found in Tn916, Tn5397 has a large resolvase gene, tndX. The C. perfringens element encodes the putative Int459 protein, which is a member of the integrase family of site-specific recombinases but is not closely related to Int from Tn916. Based on these studies it is concluded that the clostridial elements have a modular genetic organization and were derived independently from distinct mobile genetic elements.

摘要

对艰难梭菌的接合转座子Tn5397、粪肠球菌的Tn916以及产气荚膜梭菌的CW459tet(M)元件进行的比较分析表明，这些四环素抗性元件密切相关。所有这三种元件都含有tet(M)抗性基因，并且在它们的中心区域具有序列相似性。然而，它们具有非常不同的整合/切除模块。Tn5397没有Tn916中发现的int和xis基因，而是有一个大的解离酶基因tndX。产气荚膜梭菌元件编码推定的Int459蛋白，它是位点特异性重组酶整合酶家族的成员，但与Tn916的Int关系不密切。基于这些研究得出结论，梭菌元件具有模块化的遗传组织，并且独立地源自不同的可移动遗传元件。