Salustri A, Camaioni A, Tirone E, D'Alessandris C
Department of Public Health and Cell Biology, Faculty of Medicine, University of Rome Tor Vergata, Italy.
Ital J Anat Embryol. 1995;100 Suppl 1:479-84.
A factor produced by the oocytes induces in vitro FSH-treated cumulus and mural granulosa cells to synthesize hyaluronic acid. In the present study we examined hyaluronic acid synthesis by the two cell populations in vivo. After injection of hCG into PMSG-primed immature mice, cumulus and mural granulosa cells adjacent to the antrum synthesized a large amount of such glycosaminoglycan, while the outermost layers layers of mural granulosa cells did not. The results suggest that in vivo differences in hyaluronic acid synthesis between follicle cell subpopulations depend on a diffusion gradient of the oocyte factor. We also identified a proteoglycan species synthesized by cumulus cells which may contribute to hyaluronic acid organization in the intercellular spaces.
卵母细胞产生的一种因子可诱导经体外促卵泡激素（FSH）处理的卵丘细胞和壁层颗粒细胞合成透明质酸。在本研究中，我们检测了这两种细胞群体在体内合成透明质酸的情况。向经孕马血清促性腺激素（PMSG）预处理的未成熟小鼠注射人绒毛膜促性腺激素（hCG）后，卵泡腔附近的卵丘细胞和壁层颗粒细胞合成了大量这种糖胺聚糖，而壁层颗粒细胞的最外层则没有。结果表明，卵泡细胞亚群之间在体内透明质酸合成的差异取决于卵母细胞因子的扩散梯度。我们还鉴定出一种由卵丘细胞合成的蛋白聚糖，它可能有助于细胞间空间中透明质酸的组织形成。
