Pae H O, Seo W G, Oh G S, Kim N Y, Kim Y M, Kwon T O, Shin M K, Chai K Y, Chung H T
Department of Microbiology and Immunology, Wonkwang University School of Medicine and Medicinal Resources Research Center, Chonbuk, South Korea.
Immunopharmacol Immunotoxicol. 2001 Feb;23(1):25-33. doi: 10.1081/iph-100102564.
We have examined the effect of the aqueous extract of Rhodiola sachalinensis root (RSE), a traditional herbal medicine, on nitric oxide (NO) synthesis in murine fetal hepatocytes (BNL CL.2) by measuring the stable end-product nitrite and the mRNA of inducible NO synthase (iNOS). Interferon-gamma (IFN-gamma) by itself failed to induce NO synthesis in BNL CL.2 cells. RSE also did not elicit NO synthesis at concentrations up to 1,000 microg/ml, but dose- and time-dependently induced NO synthesis in the presence of IFN-gamma in BNL CL.2 cells. Whereas RSE or IFN-gamma failed to induce detectable levels of iNOS mRNA, a combination of RSE and IFN-gamma markedly induced iNOS mRNA in BNL CL.2 cells. Thus, we found that RSE triggered IFN-gamma-primed BNL CL.2 cells to synthesize NO by inducing iNOS gene expression. The capability of RSE to induce NO synthesis might be related to the therapeutic efficacy of RSE on the liver diseases.
我们通过测量稳定终产物亚硝酸盐和诱导型一氧化氮合酶（iNOS）的mRNA，研究了传统草药高山红景天根水提取物（RSE）对小鼠胎儿肝细胞（BNL CL.2）中一氧化氮（NO）合成的影响。单独的干扰素-γ（IFN-γ）未能在BNL CL.2细胞中诱导NO合成。在浓度高达1000μg/ml时，RSE也未引发NO合成，但在BNL CL.2细胞中，在IFN-γ存在的情况下，RSE剂量和时间依赖性地诱导NO合成。虽然RSE或IFN-γ未能诱导可检测水平的iNOS mRNA，但RSE和IFN-γ的组合在BNL CL.2细胞中显著诱导iNOS mRNA。因此，我们发现RSE通过诱导iNOS基因表达，触发IFN-γ预处理的BNL CL.2细胞合成NO。RSE诱导NO合成的能力可能与其对肝脏疾病的治疗效果有关。
