MacLean L L, Vinogradov E, Crump E M, Perry M B, Kay W W
Institute for Biological Sciences, National Research Council, Ottawa, Ontario Canada.
Eur J Biochem. 2001 May;268(9):2710-6. doi: 10.1046/j.1432-1327.2001.02163.x.
Flavobacterium psychrophilum, a Gram-negative bacterium, is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic infections in reared salmonids. In humans Flavobacterium spp. have been associated with neonatal meningitis and septicemia, catheter-associated bacteremia, and pneumonia. Recently, several F. psychrophilum surface molecules, including lipopolysaccharide (LPS), have been implicated in its pathogenesis and identified as potential vaccine and diagnostic candidate macromolecules. Studies on the LPS produced by the bacterium are reported herein. The structure of the antigenic O-polysaccharide contained in the LPS of F. psychrophilum was deduced by the application of analytical NMR spectroscopy, mass spectrometry, glycose and methylation analysis, and partial hydrolysis degradations, and was found to be an unbranched polymer of trisaccharide repeating units composed of L-rhamnose (L-Rhap), 2-acetamido-2-deoxy-L-fucose (L-FucpNAc) and 2-acetamido-4-((3S,5S)-3,5-dihydroxyhexanamido)-2,4-dideoxy-D-quinovose (D-Quip2NAc4NR, 2-N-acetyl-4-N-((3S,5S)-3,5-dihydroxyhexanoyl)-D-bacillosamine) (1 : 1 : 1) and having the structure: -->4)-alpha-L-FucpNAc-(1-->3)-alpha-D-Quip2NAc4NR-(1-->2)- alpha-L-Rhap-(1--> where R is (3S,5S)-CH3CH(OH)CH2CH(OH)CH2CO-.
嗜冷黄杆菌是一种革兰氏阴性菌，是虹鳟鱼苗综合征和细菌性冷水病的病原体，可在养殖的鲑科鱼类中引起败血症感染。在人类中，黄杆菌属与新生儿脑膜炎和败血症、导管相关菌血症及肺炎有关。最近，包括脂多糖（LPS）在内的几种嗜冷黄杆菌表面分子与其发病机制有关，并被确定为潜在的疫苗和诊断候选大分子。本文报道了对该细菌产生的LPS的研究。通过应用分析核磁共振光谱、质谱、糖和甲基化分析以及部分水解降解，推断出嗜冷黄杆菌LPS中所含抗原性O-多糖的结构，发现其为由L-鼠李糖（L-Rhap）、2-乙酰氨基-2-脱氧-L-岩藻糖（L-FucpNAc）和2-乙酰氨基-4-（（3S，5S）-3，5-二羟基己酰胺基）-2，4-二脱氧-D-奎诺糖（D-Quip2NAc4NR，2-N-乙酰基-4-N-（（3S，5S）-3，5-二羟基己酰基）-D-芽孢胺）（1∶1∶1）组成的三糖重复单元的无分支聚合物，其结构为：→4）-α-L-FucpNAc-（1→3）-α-D-Quip2NAc4NR-（1→2）-α-L-Rhap-（1→，其中R为（3S，5S）-CH3CH（OH）CH2CH（OH）CH2CO-。
