Dellow W J, Chambers S T, Barrell G K, Lever M, Robson R A
Department of Biochemistry, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand.
Diabetes Res Clin Pract. 2001 Jun;52(3):165-9. doi: 10.1016/s0168-8227(01)00237-6.
Diabetes mellitus subjects, type 1 and type 2, have increased glycine betaine excretion compared to normal subjects that correlated with plasma glucose and HbA(1C) concentrations. The current study was undertaken to determine whether elevated glucose concentration directly increases glycine betaine excretion in an animal model. Non-pregnant female Coopworth sheep received an intravenous glucose load (12.5,25 and 50% w/v; rate 200 ml/h) for 6 h followed by a 12 h physiological saline washout (0.9% w/v). Plasma and urine samples were analyzed for glycine betaine and glucose. Urine volumes and osmolality were also measured. Using the non-parametric Kruskal Wallis analysis of variance test we found no difference in glycine betaine excretion between glucose loaded and saline infused control animals (P=0.861). However, a significant negative correlation (r=-0.28, P<0.001) was observed between urine osmolality and glycine betaine excretion independent of treatment. We conclude that acute elevations of plasma glucose concentrations did not result in increased glycine betaine excretion and is therefore unlikely to be directly responsible for elevated glycine betaine excretion observed in diabetes mellitus subjects.
1型和2型糖尿病患者与正常受试者相比，其甜菜碱排泄量增加，且与血糖和糖化血红蛋白（HbA₁C）浓度相关。本研究旨在确定在动物模型中，血糖浓度升高是否会直接增加甜菜碱的排泄。未怀孕的雌性库泊沃斯绵羊静脉输注葡萄糖负荷（12.5%、25%和50% w/v；速率200 ml/h）6小时，随后用生理盐水冲洗12小时（0.9% w/v）。分析血浆和尿液样本中的甜菜碱和葡萄糖含量。同时测量尿量和渗透压。使用非参数Kruskal Wallis方差分析检验，我们发现输注葡萄糖的动物和输注生理盐水的对照动物之间甜菜碱排泄量没有差异（P = 0.861）。然而，无论治疗情况如何，尿量渗透压与甜菜碱排泄之间均存在显著负相关（r = -0.28，P < 0.001）。我们得出结论，血浆葡萄糖浓度的急性升高不会导致甜菜碱排泄增加，因此不太可能直接导致糖尿病患者中观察到的甜菜碱排泄增加。
