Ke Y S, Liu Z F, Yang H, Yang T, Huang J S, Rui S B, Cheng G H, Wang Y X
Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.
Acta Pharmacol Sin. 2000 Apr;21(4):345-7.
To evaluate the protective effect of anti-digoxin antiserum on hypoxia-reoxygenation induced injured myocardium and its mechanism.
Anti-digoxin antiserum of different concentrations was used, its effect on endoxin and ATPase activity in cell membrane in hypoxia-reoxygenation myocardium model was observed.
The level of endoxin was remarkably higher, ATPase activities in cell membrane were remarkably lower in hypoxic group and hypoxia-reoxygenation injury group than those of normal group; anti-digoxin antiserum could resume ATPase activity in a concentration-dependent manner.
Rise of endoxin was the molecular biological basis of myocardial damage during myocardial hypoxia-reoxygenation. Anti-digoxin antiserum had lessened myocardial injury and had a protective effect on hypoxia-reoxygenation myocardium by antagonizing effect of endoxin.
评估抗地高辛抗血清对缺氧复氧诱导的心肌损伤的保护作用及其机制。
使用不同浓度的抗地高辛抗血清，观察其对缺氧复氧心肌模型中内毒素和细胞膜ATP酶活性的影响。
缺氧组和缺氧复氧损伤组的内毒素水平显著高于正常组，细胞膜ATP酶活性显著低于正常组；抗地高辛抗血清可呈浓度依赖性恢复ATP酶活性。
内毒素升高是心肌缺氧复氧过程中心肌损伤的分子生物学基础。抗地高辛抗血清通过拮抗内毒素的作用减轻了心肌损伤，对缺氧复氧心肌具有保护作用。
