Wang De-Guo, Wang He-Gui, Ke Yong-Sheng, Yang Shang-Yin
Department of Geriatric Cardiology, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Feb;23(1):9-12.
To study the effect of antidigoxin antiserum on oxygen stress induced by myocardial ischemia/reperfusion (MI/R) injury in rats.
Sprauge Dawley rats were submitted to ligate left anterior descending coronary artery 30 min followed by 45 min reperfusion. Experiment animals were randomly divided into seven groups including sham group, MI/R group, normal salina group, verapamil group and three antidigoxin antiserum groups from low to high dose. The left ventricular myocardial tissue sample of ischemia were processed and measured the level of endoxin and malondialdehyde (MDA), the activities of Na+, K(+) -ATPase and superoxin dismutase (SOD). The myocardia morphology was observed.
The levels of endoxin and MDA increased and the activities of Na+, K(+) -ATPase and MDA were inhibited significantly in MI/R and saline groups. Including verapamil group in comparison to MI/R and saline groups, MDA level decreased and SOD activities partly reserved, meanwhile, only in three antidigoxin antiserum groups, the myocardial endoxin level was remarkably decreased, Na+, K(+) -ATPase activities were drastically increased. The myocardial histological morphology was significantly improved.
Antidigoxin antiserum, an endoxin mutual clone antibody, had the effect of attenuating the damage of oxygen free radicals induced by MI/R via to antagonizing the inhibition effect of endoxin on myocardial membrane Na+, K(+) -ATPase activities.
研究抗地高辛抗血清对大鼠心肌缺血/再灌注（MI/R）损伤所致氧化应激的影响。
将Sprauge Dawley大鼠左冠状动脉前降支结扎30分钟，随后再灌注45分钟。实验动物随机分为七组，包括假手术组、MI/R组、生理盐水组、维拉帕米组以及三个低、中、高剂量抗地高辛抗血清组。对缺血的左心室心肌组织样本进行处理，检测内毒素和丙二醛（MDA）水平、Na⁺，K⁺-ATP酶和超氧化物歧化酶（SOD）活性，并观察心肌形态。
MI/R组和生理盐水组中，内毒素和MDA水平升高，Na⁺，K⁺-ATP酶和MDA活性显著受到抑制。与MI/R组和生理盐水组相比，维拉帕米组MDA水平降低，SOD活性部分恢复，同时，仅在三个抗地高辛抗血清组中，心肌内毒素水平显著降低，Na⁺，K⁺-ATP酶活性大幅升高，心肌组织形态显著改善。
抗地高辛抗血清作为一种内毒素单克隆抗体，通过拮抗内毒素对心肌膜Na⁺，K⁺-ATP酶活性的抑制作用，具有减轻MI/R所致氧自由基损伤的作用。
