Kang J H, Shi Y M, Zheng R L
Department of Biology, Lanzhou University, Lanzhou 730000, China.
Acta Pharmacol Sin. 2000 Apr;21(4):348-52.
To examine the effects of ascorbic acid (AA) and DL-alpha-tocopherol (alpha T) on the proliferation and redifferentiation of human hepatoma cell.
Choosing an all-trans retinoic acid (RA) as a positive control, cell surface charge, biochemical changes, and cell growth in soft agar were measured.
After treatment with AA 4 mmol.L-1 and alpha T 1 mmol.L-1 together, the growth curve and mitotic index of human hepatoma cells decreased remarkably, the cellular growth inhibitory rate amounted to 61.3%. The indices related to cell malignancy alleviated significantly; cell surface charge decreased, the electrophoresis rate dropped from 1.64 to 0.89 microns.s-1.V-1.cm-1, the average value of alpha-fetoprotein (alpha-FP) content decreased from 300 to 80 micrograms.g-1 (protein), and gamma-glutamyl-transpeptidase (gamma-GT) activity decreased from 0.81 to 0.201 U.g-1(protein). The index related to cell differentiation increased significantly, such as the average level of tyrosine-alpha-ketoglutarate transaminase activity increased from 10.6 to 45 micrograms.g-1 (protein), and the colonogenic potential decreased by 96.6%.
AA and alpha T combination inhibited human hepatoma cell proliferation, induced redifferentiation, and reversed its malignant phenotypic characteristics.
研究抗坏血酸（AA）和DL-α-生育酚（αT）对人肝癌细胞增殖和再分化的影响。
选用全反式维甲酸（RA）作为阳性对照，检测细胞表面电荷、生化变化及软琼脂中的细胞生长情况。
用4 mmol.L-1的AA和1 mmol.L-1的αT共同处理后，人肝癌细胞的生长曲线和有丝分裂指数显著下降，细胞生长抑制率达61.3%。与细胞恶性程度相关的指标明显改善；细胞表面电荷降低，电泳速率从1.64降至0.89微米·秒-1·伏-1·厘米-1，甲胎蛋白（α-FP）含量平均值从300降至80微克·克-1（蛋白质），γ-谷氨酰转肽酶（γ-GT）活性从0.81降至0.201单位·克-1（蛋白质）。与细胞分化相关的指标显著升高，如酪氨酸-α-酮戊二酸转氨酶活性平均水平从10.6升至45微克·克-1（蛋白质），集落形成能力降低96.6%。
AA与αT联合应用可抑制人肝癌细胞增殖，诱导再分化，并逆转其恶性表型特征。
