Kang J H, Shi Y M, Zheng R L
Department of Biology, Lanzhou University, Lanzhou 730000, China.
Zhongguo Yao Li Xue Bao. 1999 Nov;20(11):1019-24.
To examine the effects of ascorbic acid (AA) on hepatoma.
Choosing an all-trans tretinoin (Tre) as a positive control, cell growth, and cell redifferentiation tests by cell surface charges, biochemical changes, and cell growth in soft agar were measured.
After being treated with AA 6 mmol.L-1, the growth curve and mitotic index of human hepatoma cells decreased remarkably, the cellular growth inhibitory rate amounted to 58.9%. The indices related with cell malignancy alleviated, such as cell surface charge obviously decreased, the electrophoresis rate dropped from 1.64 microns.s-1.V-1.cm-1 to 0.93, the average value of alpha-fetoprotein (alpha-FP) content decreased from 302 micrograms.g-1(protein) to 90, and gamma-glytamyl-transpeptidase (gamma-GT) activity from 0.81 U.g-1(protein) to 0.16. The index related with cell differentiation increased, such as the average level of tyrosine-alpha-ketoglutarate transminase activity increased from 10.3 micromol.g-1(protein) to 41.2, and the colonogenic potential decreased 94.4%.
AA can inhibit human hepatoma cells proliferation, induce redifferentiation, and reverse its malignant phenotypic characteristics.
研究抗坏血酸（AA）对肝癌的影响。
选用全反式维甲酸（Tre）作为阳性对照，通过细胞表面电荷、生化变化及软琼脂中的细胞生长情况进行细胞生长和细胞再分化试验。
用6 mmol.L-1 AA处理后，人肝癌细胞的生长曲线和有丝分裂指数显著下降，细胞生长抑制率达58.9%。与细胞恶性程度相关的指标有所改善，如细胞表面电荷明显降低，电泳速率从1.64微米·秒-1·伏-1·厘米-1降至0.93，甲胎蛋白（α-FP）含量平均值从302微克·克-1（蛋白质）降至90，γ-谷氨酰转肽酶（γ-GT）活性从0.81 U·克-1（蛋白质）降至0.16。与细胞分化相关的指标有所增加，如酪氨酸-α-酮戊二酸转氨酶活性平均水平从10.3微摩尔·克-1（蛋白质）增至41.2，集落形成能力下降94.4%。
AA可抑制人肝癌细胞增殖，诱导再分化，并逆转其恶性表型特征。
