Zhao F, Yuan Y, Tan L, Zhang X
General Hospital of Nanjing Command, PLA, Nanjing, 210002.
Se Pu. 1998 Jan;16(1):78-9.
A simple and rapid method for the determination of tranilast in human plasma by RP-HPLC is described. The analytical column was packed with YWG-C18. The mobile phase consisted of methanol-0.02 mol/L KH2PO4(60:40, V/V; pH 4.2), and detected by UV detector at a wavelength of 333 nm. The plasma sample was injected directly into the HPLC system after precipitation of the protein with methanol. The calibration curve was linear within the concentration range of 0.625-40 mg/L. The average recovery was 100.0% +/- 4.1%, and minimum detectable concentration was 0.2 mg/L. By using this method, the pharmacokinetics of tranilast in the plasma of ten healthy volunteers after oral administration of 200 mg drug were studied. The plasma drug concentration-time curve of volunteers conforms to one-compartment open model.
描述了一种用反相高效液相色谱法测定人血浆中曲尼司特的简单快速方法。分析柱填充YWG-C18。流动相由甲醇-0.02mol/L磷酸二氢钾（60:40，V/V；pH4.2）组成，用紫外检测器在333nm波长处检测。血浆样品用甲醇沉淀蛋白质后直接注入高效液相色谱系统。校准曲线在0.625 - 40mg/L浓度范围内呈线性。平均回收率为100.0%±4.1%，最低检测浓度为0.2mg/L。采用该方法研究了10名健康志愿者口服200mg药物后血浆中曲尼司特的药代动力学。志愿者的血浆药物浓度-时间曲线符合一室开放模型。
