Tan L, Xia Y B, Tu X D, Yuan Y S
Department of Instrumental Analysis, Nanjing General Hospital of Nanjing Command.
Yao Xue Xue Bao. 1994;29(3):232-6.
Following detailed study, a rapid and sensitive assay for ethmozine in human plasma has been developed using reversed phase high performance liquid chromatography (HPLC). Plasma samples were prepared for analysis by addition of internal standard (5-chloro-2-amino-benzophenone) followed by protein precipitation using acetonitrile. Analytical column was a C18 Spherisorb. The mobile phase consisted of mathanol-water-triethylamine (70:30:0.4, v/v/v, pH 6.5). The column effluent was monitored at 268 nm. The calibration curve was linear in the range from 20 ng/ml to 4000 ng/ml with r = 0.9994. The detection limit of this method was 3 ng/ml. The method showed good precision and the analytical recovery of ethmozine from plasma was 90-105%. The relative standard deviations for within-day and between-day were 2.4-6.3% and 4.5-10.2% respectively. The plasma drug concentration-time course in man after oral administration of 400 mg after conformed to a 1-compartment open model with a first order absorption phase. Mean T1/2 value was 1.75 +/- 0.45 h.
经过详细研究，已开发出一种使用反相高效液相色谱法（HPLC）测定人血浆中乙吗噻嗪的快速灵敏分析方法。血浆样品通过添加内标（5-氯-2-氨基二苯甲酮）进行分析前处理，随后用乙腈进行蛋白沉淀。分析柱为C18 Spherisorb。流动相由甲醇 - 水 - 三乙胺（70:30:0.4，v/v/v，pH 6.5）组成。在268 nm处监测柱流出物。校准曲线在20 ng/ml至4000 ng/ml范围内呈线性，r = 0.9994。该方法的检测限为3 ng/ml。该方法具有良好的精密度，乙吗噻嗪从血浆中的分析回收率为90 - 105%。日内和日间的相对标准偏差分别为2.4 - 6.3%和4.5 - 10.2%。口服400 mg后人体血浆药物浓度 - 时间过程符合具有一级吸收相的单室开放模型。平均T1/2值为1.75 +/- 0.45小时。
