Nilsson E, Parrott J A, Skinner M K
Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99163-4231, USA.
Mol Cell Endocrinol. 2001 Apr 25;175(1-2):123-30. doi: 10.1016/s0303-7207(01)00391-4.
The recruitment of primordial follicles to initiate folliculogenesis determines the population of developing follicles available for ovulation and directly regulates female reproductive efficiency. In the current study, a floating organ culture system was used to examine the progression of primordial (stage 0) follicles to developing (stages 1-4) follicles in 4-day-old pre-pubertal rat ovaries. Basic fibroblast growth factor (bFGF) was found to induce primordial follicle development similar to what has been demonstrated for kit ligand/stem cell factor (KL). The bFGF-treated ovaries contained 85% developing follicles compared with 50% developing follicles for control untreated organ cultures. Correspondingly, the number of primordial follicles in bFGF-treated ovaries decreased to 15% of the total compared with 45% for controls. A bFGF neutralizing antibody was found to decrease the small amount of spontaneous follicle development that occurs during the organ culture. Basic FGF was localized to primordial and early developing follicles by immunocytochemistry and was primarily observed in the oocytes. Treatment of bovine ovarian theca cells and stroma cells with bFGF was found to promote cell growth. Basic FGF produced by the oocyte in early stage follicles appears to act on adjacent somatic cells to promote cell growth and development. Basic FGF, like KL, appears to be a primordial follicle-inducing factor. In summary, bFGF can regulate primordial follicle development that directly influences female reproductive efficiency.
原始卵泡的募集以启动卵泡发生过程，这决定了可供排卵的发育中卵泡的数量，并直接调节雌性生殖效率。在本研究中，采用悬浮器官培养系统来检测4日龄青春期前大鼠卵巢中原始（0期）卵泡向发育中（1-4期）卵泡的进展情况。发现碱性成纤维细胞生长因子（bFGF）可诱导原始卵泡发育，这与已证明的kit配体/干细胞因子（KL）的作用相似。与未处理的对照器官培养物中50%的发育中卵泡相比，经bFGF处理的卵巢中含有85%的发育中卵泡。相应地，经bFGF处理的卵巢中原始卵泡的数量降至总数的15%，而对照组为45%。发现一种bFGF中和抗体可减少器官培养过程中发生的少量自发卵泡发育。通过免疫细胞化学方法将碱性成纤维细胞生长因子定位到原始卵泡和早期发育卵泡，并且主要在卵母细胞中观察到。发现用bFGF处理牛卵巢膜细胞和基质细胞可促进细胞生长。早期卵泡中的卵母细胞产生的碱性成纤维细胞生长因子似乎作用于相邻的体细胞以促进细胞生长和发育。碱性成纤维细胞生长因子与KL一样，似乎是一种原始卵泡诱导因子。总之，bFGF可调节直接影响雌性生殖效率的原始卵泡发育。
