Domínguez-Bello M G, Cienfuentes C, Romero R, García P, Gómez I, Mago V, Reyes N, Gueneau de Novoa P
Instituto Venezolano de Investigaciones Cientificas, CBB, Caracas, Venezuela.
FEMS Microbiol Lett. 2001 Apr 20;198(1):15-6. doi: 10.1111/j.1574-6968.2001.tb10612.x.
Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.
检测幽门螺杆菌感染的分子方法在胃活检和培养中已显示出高度敏感性。这项工作的目的是比较在经线吸收的胃液和胃活检中幽门螺杆菌DNA的PCR检测。该研究在47名接受内镜检查的消化不良成年患者中进行，通过扩增幽门螺杆菌ureA基因的一段来检测感染。在活检分析呈阳性的29名患者中，23名（79%）经胃线检测也呈阳性。当不进行内镜检查时，对胃线进行PCR分析是检测幽门螺杆菌的一种敏感且安全的方法，在确定根除治疗的有效性、对毒力基因分型以及检测抗生素耐药性突变方面可能具有很大的临床和流行病学用途。
