特布他林对大鼠变应性炎症中神经生长因子形成的影响。

Effects of terbutaline on NGF formation in allergic inflammation of the rat.

作者信息

Amann R, Peskar B A, Schuligoi R

机构信息

Institute of Experimental & Clinical Pharmacology, University of Graz, Univ.-Platz 4, A-8010 Graz, Austria.

出版信息

Br J Pharmacol. 2001 May;133(1):186-92. doi: 10.1038/sj.bjp.0704060.

DOI:10.1038/sj.bjp.0704060

PMID:11325809

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1572771/

Abstract

The aim of this study was to determine the effects of the beta adrenergic agonist terbutaline on NGF increase caused by allergic inflammation in rats. 2. Intraplantar antigen injection in sensitized rats increased paw volume and stimulated NGF biosynthesis in the skin of the injected paw as determined 3 and 6 h after injection. Treatment of rats with terbutaline (0.1 - 0.3 mg kg(-1), s.c.) had no significant effect on the NGF concentration in non-inflamed skin, but reduced oedema, and at 0.3 mg kg(-1) also NGF mRNA and immunoreactive NGF in the skin of the inflamed paw in a propranolol-reversible manner. In carrageenan-induced inflammation, terbutaline did not significantly reduce the inflammation-induced increase of NGF in paw skin. 3. Exposure of sensitized rats to aerosolized antigen (twice, 24 h interval) increased protein content, eosinophil leukocytes, and immunoreactive NGF in the bronchoalveolar lavage fluid (BAL, obtained 16 h after the second antigen exposure). Treatment of rats with terbutaline (0.3 mg kg(-1), s.c. 30 min before the second antigen challenge) suppressed antigen-induced elevation of protein and eosinophil leukocytes, and reduced the concentration of NGF in BAL to values similar to those found in non-sensitized rats. 4. The present results demonstrate anti-allergic properties of terbutaline in rats that were accompanied by a marked reduction of antigen-induced NGF increase in skin and BAL, respectively. These results are compatible with the assumption that terbutaline primarily suppressed the immune response to antigen thereby attenuating the release of vasoactive mediators and the stimulation of NGF biosynthesis.

摘要

本研究的目的是确定β肾上腺素能激动剂特布他林对大鼠过敏性炎症引起的神经生长因子（NGF）增加的影响。2. 在致敏大鼠足底注射抗原后，如在注射后3小时和6小时所测定的，注射侧爪体积增加，且注射侧爪皮肤中的NGF生物合成受到刺激。用特布他林（0.1 - 0.3 mg kg⁻¹，皮下注射）处理大鼠，对非炎症皮肤中的NGF浓度无显著影响，但可减轻水肿，且在剂量为0.3 mg kg⁻¹时，还能以普萘洛尔可逆的方式降低炎症爪皮肤中的NGF mRNA和免疫反应性NGF。在角叉菜胶诱导的炎症中，特布他林未显著降低炎症诱导的爪皮肤中NGF的增加。3. 使致敏大鼠暴露于雾化抗原（两次，间隔24小时），可增加支气管肺泡灌洗液（BAL，在第二次抗原暴露后16小时获得）中的蛋白质含量、嗜酸性粒细胞以及免疫反应性NGF。用特布他林（0.3 mg kg⁻¹，在第二次抗原攻击前30分钟皮下注射）处理大鼠，可抑制抗原诱导的蛋白质和嗜酸性粒细胞升高，并将BAL中的NGF浓度降低至与未致敏大鼠相似的值。4. 目前的结果表明，特布他林在大鼠中具有抗过敏特性，同时分别伴随着抗原诱导的皮肤和BAL中NGF增加的显著减少。这些结果与以下假设相符，即特布他林主要抑制对抗原的免疫反应，从而减弱血管活性介质的释放和NGF生物合成的刺激。