Arrell D K, Neverova I, Van Eyk J E
Departments of Physiology, Queen's University, Kingston, Ontario, Canada.
Circ Res. 2001 Apr 27;88(8):763-73. doi: 10.1161/hh0801.090193.
The development of proteomics is a timely one for cardiovascular research. Analyses at the organ, subcellular, and molecular levels have revealed dynamic, complex, and subtle intracellular processes associated with heart and vascular disease. The power and flexibility of proteomic analyses, which facilitate protein separation, identification, and characterization, should hasten our understanding of these processes at the protein level. Properly applied, proteomics provides researchers with cellular protein "inventories" at specific moments in time, making it ideal for documenting protein modification due to a particular disease, condition, or treatment. This is accomplished through the establishment of species- and tissue-specific protein databases, providing a foundation for subsequent proteomic studies. Evolution of proteomic techniques has permitted more thorough investigation into molecular mechanisms underlying cardiovascular disease, facilitating identification not only of modified proteins but also of the nature of their modification. Continued development should lead to functional proteomic studies, in which identification of protein modification, in conjunction with functional data from established biochemical and physiological methods, has the ability to further our understanding of the interplay between proteome change and cardiovascular disease.
蛋白质组学的发展对心血管研究来说恰逢其时。在器官、亚细胞和分子水平上的分析揭示了与心脏和血管疾病相关的动态、复杂且微妙的细胞内过程。蛋白质组学分析的强大功能和灵活性有助于蛋白质的分离、鉴定和表征，应能加速我们在蛋白质水平上对这些过程的理解。如果应用得当，蛋白质组学能在特定时刻为研究人员提供细胞蛋白质“清单”，这使其成为记录因特定疾病、状况或治疗导致的蛋白质修饰的理想方法。这是通过建立物种和组织特异性蛋白质数据库来实现的，为后续的蛋白质组学研究奠定基础。蛋白质组学技术的发展使得对心血管疾病潜在分子机制的研究更加深入，不仅有助于鉴定修饰的蛋白质，还能确定其修饰的性质。持续的发展应能导向功能蛋白质组学研究，在这类研究中，蛋白质修饰的鉴定与来自既定生化和生理方法的功能数据相结合，将有助于我们进一步理解蛋白质组变化与心血管疾病之间的相互作用。
