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蛋白质组学分析确定了过氧化物酶体增殖物激活受体2与患心肌病仓鼠的早期左心室损伤有关。

Proteomics analysis identified peroxiredoxin 2 involved in early-phase left ventricular impairment in hamsters with cardiomyopathy.

作者信息

Kuzuya Kentaro, Ichihara Sahoko, Suzuki Yuka, Inoue Chisa, Ichihara Gaku, Kurimoto Syota, Oikawa Shinji

机构信息

Department of Human Functional Genomics, Life Science Research Center, Mie University, Tsu, Japan.

Graduate School of Regional Innovation Studies, Mie University, Tsu, Japan.

出版信息

PLoS One. 2018 Feb 13;13(2):e0192624. doi: 10.1371/journal.pone.0192624. eCollection 2018.

DOI:10.1371/journal.pone.0192624
PMID:29438398
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5810987/
Abstract

Given the hypothesis that inflammation plays a critical role in the progression of cardiovascular diseases, the aim of the present study was to identify new diagnostic and prognostic biomarkers of myocardial proteins involved in early-phase cardiac impairment, using proteomics analysis. Using the two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined with MALDI-TOF/TOF tandem mass spectrometry, we compared differences in the expression of proteins in the whole left ventricles between control hamsters, dilated cardiomyopathic hamsters (TO-2), and hypertrophy cardiomyopathic hamsters (Bio14.6) at 6 weeks of age (n = 6, each group). Proteomic analysis identified 10 protein spots with significant alterations, with 7 up-regulated and 3 down-regulated proteins in the left ventricles of both TO-2 and Bio 14.6 hamsters, compared with control hamsters. Of the total alterations, peroxiredoxin 2 (PRDX2) showed significant upregulation in the left ventricles of TO-2 and Bio 14.6 hamsters. Our data suggest that PRDX2, a redox regulating molecule, is involved in early-phase left ventricular impairment in hamsters with cardiomyopathy.

摘要

鉴于炎症在心血管疾病进展中起关键作用这一假说，本研究的目的是通过蛋白质组学分析，鉴定参与早期心脏损伤的心肌蛋白的新诊断和预后生物标志物。使用二维荧光差异凝胶电泳（2D-DIGE）结合基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF），我们比较了6周龄对照仓鼠、扩张型心肌病仓鼠（TO-2）和肥厚型心肌病仓鼠（Bio14.6）（每组n = 6）整个左心室中蛋白质表达的差异。蛋白质组学分析确定了10个有显著变化的蛋白点，与对照仓鼠相比，TO-2和Bio 14.6仓鼠左心室中有7个蛋白上调，3个蛋白下调。在所有变化中，过氧化物酶2（PRDX2）在TO-2和Bio 14.6仓鼠的左心室中显示出显著上调。我们的数据表明，氧化还原调节分子PRDX2参与了仓鼠心肌病早期左心室损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/c565755713a5/pone.0192624.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/ca682d2c92fb/pone.0192624.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/b790ceaca75f/pone.0192624.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/4b7ab25f5723/pone.0192624.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/19c24e94b874/pone.0192624.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/6cdff26930ea/pone.0192624.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/c565755713a5/pone.0192624.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/ca682d2c92fb/pone.0192624.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/b790ceaca75f/pone.0192624.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/4b7ab25f5723/pone.0192624.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/19c24e94b874/pone.0192624.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/6cdff26930ea/pone.0192624.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fb/5810987/c565755713a5/pone.0192624.g006.jpg

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