Yang Z H, Bates J H, Eisenach K D, Cave M D
Regional Tuberculosis Genotyping Laboratory, Central Arkansas Veterans Healthcare System, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
J Clin Microbiol. 2001 May;39(5):1691-5. doi: 10.1128/JCM.39.5.1691-1695.2001.
Fifty-nine isolates of Mycobacterium tuberculosis obtained from different states in the United States and representing 25 interstate clusters were investigated. These clusters were identified by computer-assisted analysis of DNA fingerprints submitted during 1996 and 1997 by different laboratories participating in the CDC National Genotyping and Surveillance Network. Isolates were fingerprinted with the IS6110 right-hand probe (IS6110-3'), the IS6110 left-hand probe (IS6110-5'), and the probe pTBN12, containing the polymorphic GC-rich sequence (PGRS). Spoligotyping based on the polymorphism in the 36-bp direct-repeat locus was also performed. As a control, 43 M. tuberculosis isolates in 17 clusters obtained from patients in Arkansas during the study period were analyzed. Of the 25 interstate clusters, 19 were confirmed as correctly clustered when all the isolates were analyzed on the same gel using the IS6110-3' probe. Of the 19 true IS6110-3' clusters, 10 (53%) were subdivided by one or more secondary typing methods. Clustering of the control group was virtually identical by all methods. Of the three different secondary typing methods, spoligotyping was the least discriminating. IS6110-5' fingerprinting was as discriminating as PGRS fingerprinting. The data indicate that the IS6110-5' probe not only is a useful secondary typing method but also probably would prove to be a more useful primary typing method for a genotyping network which involves isolates from different geographic regions.
对从美国不同州获取的59株结核分枝杆菌分离株进行了研究，这些分离株代表了25个跨州集群。这些集群是通过对参与疾病控制与预防中心（CDC）国家基因分型和监测网络的不同实验室在1996年和1997年提交的DNA指纹进行计算机辅助分析而确定的。使用IS6110右手探针（IS6110 - 3'）、IS6110左手探针（IS6110 - 5'）以及包含多态性富含GC序列（PGRS）的探针pTBN12对分离株进行指纹分析。还基于36 bp直接重复位点的多态性进行了间隔寡核苷酸分型（Spoligotyping）。作为对照，对研究期间从阿肯色州患者中获得的17个集群中的43株结核分枝杆菌分离株进行了分析。在25个跨州集群中，当使用IS6110 - 3'探针在同一凝胶上分析所有分离株时，19个被确认为正确聚类。在这19个真正的IS6110 - 3'集群中，10个（53%）通过一种或多种二级分型方法进行了细分。所有方法对对照组的聚类结果几乎相同。在三种不同的二级分型方法中，间隔寡核苷酸分型的鉴别力最低。IS6110 - 5'指纹分析与PGRS指纹分析的鉴别力相当。数据表明，IS6110 - 5'探针不仅是一种有用的二级分型方法，而且对于一个涉及来自不同地理区域分离株的基因分型网络来说，可能也会被证明是一种更有用的一级分型方法。
