Yabuki A, Suzuki S, Matsumoto M, Nishinakagawa H
Department of Veterinary Anatomy, Faculty of Agriculture, Kagoshima University, 21-24 Korimoto 1, Kagoshima-shi, Kagoshima 890-0065, Japan.
Exp Anim. 2001 Jan;50(1):59-66. doi: 10.1538/expanim.50.59.
We recently demonstrated sexual dimorphism in the S3 segment of the ICR mouse kidney, as differences in periodic acid Schiff (PAS) staining on the brush border and the number and size of PAS-positive granules. However, whether these sex dependent features in the S3 segment of the mouse kidney occur only in the ICR strain or are a general feature also observed in other strains is unclear. In the present study, we examined the renal S3 segment of the ICR, BALB/c, C57BL/6, C3H/He and DBA/2 mice strains, which are commonly used in laboratory experiments. PAS staining of the brush border in females of all strains was more intense than that of males, and PAS-positive granules were detected in all females. In male groups, PAS-positive granules were detected in the DBA/2 strain only, but their number was very few. In addition, PAS-positive giant bodies, larger than the nuclear size, were detected in females except those of the C57BL/6 strain. Histometrical investigation demonstrated apparent strain differences in a number of PAS-positive granules and PAS-positive giant bodies. The ultrastructural and cytochemical investigations suggest that the PAS-positive granules and PAS-positive giant bodies were multilamellar lysosomes. We propose that the present findings are significant for comparative morphology in laboratory animal science.
我们最近在ICR小鼠肾脏的S3段证实了性别二态性，表现为刷状缘高碘酸希夫（PAS）染色以及PAS阳性颗粒数量和大小存在差异。然而，小鼠肾脏S3段中这些依赖性别的特征是仅在ICR品系中出现，还是在其他品系中也普遍存在，目前尚不清楚。在本研究中，我们检查了常用于实验室实验的ICR、BALB/c、C57BL/6、C3H/He和DBA/2小鼠品系的肾脏S3段。所有品系雌性小鼠刷状缘的PAS染色均比雄性更强烈，并且在所有雌性小鼠中均检测到PAS阳性颗粒。在雄性组中，仅在DBA/2品系中检测到PAS阳性颗粒，但其数量非常少。此外，除C57BL/6品系外，在其他雌性小鼠中均检测到大于细胞核大小的PAS阳性巨大体。组织计量学研究表明，PAS阳性颗粒和PAS阳性巨大体的数量存在明显的品系差异。超微结构和细胞化学研究表明，PAS阳性颗粒和PAS阳性巨大体是多层溶酶体。我们认为，本研究结果对实验动物科学中的比较形态学具有重要意义。
