Kadota H, Kuwahara M, Nishibata R, Mikami H, Tsubone H
Department of Comparative Pathophysiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Exp Anim. 2001 Jan;50(1):91-5. doi: 10.1538/expanim.50.91.
To clarify the effect of acetylcholinesterase (AChE) on the pathogenesis of airway hyperresponsiveness, AChE activities in tracheal smooth muscle and lung tissue from congenitally bronchial-hypersensitive (BHS) and bronchial-hyposensitive (BHR) guinea pigs were compared. For this purpose, AChE activities were determined by measuring the rate of absorbance of tissue homogenate. Relative amounts of AChE mRNA were also evaluated by the RT-PCR method. In both tracheal smooth muscle and lung tissue from BHS, the AChE activity and the relative amount of AChE mRNA were less than those in BHR. These results suggest that the reduced AChE activity is at least a candidate for inducing airway hyperresponsiveness.
为阐明乙酰胆碱酯酶（AChE）在气道高反应性发病机制中的作用，比较了先天性支气管高敏（BHS）和支气管低敏（BHR）豚鼠气管平滑肌和肺组织中的AChE活性。为此，通过测量组织匀浆的吸光度速率来测定AChE活性。还采用逆转录聚合酶链反应（RT-PCR）法评估AChE mRNA的相对含量。在BHS豚鼠的气管平滑肌和肺组织中，AChE活性及AChE mRNA的相对含量均低于BHR豚鼠。这些结果表明，AChE活性降低至少是诱导气道高反应性的一个可能因素。
