Wang C, Zou G
Department of Chemistry, Nanjing University, Nanjing, 210093.
Se Pu. 1998 May;16(3):255-7.
A reversed-phase HPLC method has been developed for the separation and determination of naproxen and its bromo-substituted compound. These compounds were separated on a Shim-pack CLC ODS column (5 microns, 250 mm x 4.6 mm i.d.) by using a methanol-water (80/20, V/V) solution containing 50 mmol/L lactic acid and adjusted to pH 2.5 with perchloric acid as mobile phase, with flow rate of 0.8 mL/min and UV detection at 271 nm. Benzoic acid was selected as an internal standard. At the concentration range of 5-100 mg/L, the linear internal standard working curves with r > 0.9995 were obtained. The accuracy of this method for naproxen and its bromo-substituted compound were 99.83%-102.07% and 99.00%-100.83%, and RSD were < 2.58% and < 3.64% respectively.
已开发出一种反相高效液相色谱法用于分离和测定萘普生及其溴代取代化合物。这些化合物在Shim-pack CLC ODS柱（5微米，250毫米×4.6毫米内径）上进行分离，以含50毫摩尔/升乳酸且用高氯酸调至pH 2.5的甲醇-水（80/20，V/V）溶液为流动相，流速为0.8毫升/分钟，在271纳米处进行紫外检测。选用苯甲酸作为内标。在5 - 100毫克/升的浓度范围内，得到了线性内标工作曲线，r > 0.9995。该方法对萘普生及其溴代取代化合物的准确度分别为99.83% - 102.07%和99.00% - 100.83%，相对标准偏差分别< 2.58%和< 3.64%。
