Zeng X J, Zhang N Z
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and PUMC, Beijing 100730.
Yao Xue Xue Bao. 1997;32(3):171-3.
Tripchlorolide(T4) is an active ingredient recently isolated from Tripterygium Wilfordii Hook. The in vitro effects of T4 on the peripheral blood mononuclear cells(PBMC) of healthy persons(n = 10), the digested single synovium cells(DSSC) (n = 3) and PBMC(n = 6) from RA patients on production of immunoglobulins (Ig) were studied using Elisa method. The results showed that T4 at concentrations from 5 ng.ml-1 to 35 ng.ml-1 significantly reduced the production of Ig by PWM-stimulated PBMC of healthy persons in a dose dependent manner. At concentration of 25 ng.ml-1, T4 also reduced the Ig secretion of RA-PBMC and DSSC. It is hoped that T4 would be an encouraging new drug of herbal nature for the treatment of RA.
雷公藤甲素（T4）是最近从雷公藤中分离出的一种活性成分。采用酶联免疫吸附测定法（ELISA法）研究了T4对10名健康人的外周血单个核细胞（PBMC）、3例类风湿关节炎（RA）患者的消化单个滑膜细胞（DSSC）和6例RA患者的PBMC产生免疫球蛋白（Ig）的体外作用。结果显示，浓度为5 ng/ml至35 ng/ml的T4能以剂量依赖方式显著降低健康人经美洲商陆有丝分裂原（PWM）刺激的PBMC产生Ig。在浓度为25 ng/ml时，T4也降低了RA患者PBMC和DSSC的Ig分泌。有望T4成为一种令人鼓舞的新型天然草药药物用于治疗RA。
