Paterson S, McLachlan-Troup N, Cordero R, Dohnal M, Carman S
Toxicology Unit, Imperial College School of Medicine, London, United Kingdom.
J Anal Toxicol. 2001 Apr;25(3):203-8. doi: 10.1093/jat/25.3.203.
A previously described method for the analysis of hair has been modified to include analysis for amphetamines, benzodiazepines, cocaine and its metabolites, methadone and its metabolite, and phencyclidine in addition to opiates on a sample of hair. The samples of hair were washed twice with dichloromethane and cut into 1-mm segments prior to extraction with methanol at 45 degrees C for 18 h. The extracts were split into two parts; both were evaporated to dryness. One half of the extract was derivatized using MBTFA for analysis of amphetamines, and the other half was derivatized using MTBSTFA for analysis of the remaining drugs. The extracts were analyzed using electron impact gas chromatography-mass spectrometry operating in selected ion monitoring mode. In total, 18 drugs of abuse/metabolites could be detected. The method was used to screen 20 hair samples from patients attending a methadone-maintenance clinic.
一种先前描述的毛发分析方法已被改进，除了对毛发样本中的阿片类药物进行分析外，还包括对苯丙胺类、苯二氮䓬类、可卡因及其代谢物、美沙酮及其代谢物以及苯环利定进行分析。毛发样本先用二氯甲烷洗涤两次，切成1毫米的片段，然后在45℃下用甲醇提取18小时。提取物分成两部分；两者都蒸发至干。提取物的一半用MBTFA衍生化用于分析苯丙胺类，另一半用MTBSTFA衍生化用于分析其余药物。提取物采用在选择离子监测模式下运行的电子轰击气相色谱-质谱联用仪进行分析。总共可以检测出18种滥用药物/代谢物。该方法用于对来自美沙酮维持治疗诊所患者的20份毛发样本进行筛查。
