Carrington C A, dos Santos Cruz G
Imutran Ltd (A Novartis Pharma AG Co.), PO Box 399, Cambridge CB2 2YP, UK.
Xenotransplantation. 2001 May;8(2):100-5. doi: 10.1034/j.1399-3089.2001.00086.x.
Cultures of hDAF transgenic porcine aortic endothelial cells (TPAEs) with levels of cell surface hDAF expression between 2000 and 300 000 molecules per cell have been used to determine the relationship between expression of hDAF and protection from human complement deposition in an in vitro model. At concentrations below 45 000 molecules per cell, the relationship between hDAF expression and degree of protection conferred is linear. Concentrations of 123 000 molecules per cell and higher give maximal protection (60% reduction of susceptibility to neat human serum) in this model. It is concluded that increasing hDAF expression above that displayed by the A74 line of hDAF transgenic pigs (240 000 +/- 15 000 molecules per cell) would not confer any additional benefit.
已使用每细胞表面hDAF表达水平在2000至300000个分子之间的hDAF转基因猪主动脉内皮细胞（TPAEs）培养物，在体外模型中确定hDAF表达与免受人类补体沉积之间的关系。在每细胞低于45000个分子的浓度下，hDAF表达与所赋予的保护程度之间的关系是线性的。在该模型中，每细胞123000个分子及更高的浓度可提供最大保护（对纯人类血清的敏感性降低60%）。得出的结论是，将hDAF表达提高到高于hDAF转基因猪A74系所显示的水平（每细胞240000±15000个分子）不会带来任何额外益处。
