通过PCR-ELISA检测不同宫颈病变中特定类型人乳头瘤病毒（HPV）的分布及病毒载量。
Distribution and viral load of type specific HPVs in different cervical lesions as detected by PCR-ELISA.
作者信息
Zerbini M, Venturoli S, Cricca M, Gallinella G, De Simone P, Costa S, Santini D, Musiani M
机构信息
Department of Clinical and Experimental Medicine, Division of Microbiology, Osp.S.Orsola, University of Bologna, Via Massarenti 9, I 40138, Bologna, Italy.
出版信息
J Clin Pathol. 2001 May;54(5):377-80. doi: 10.1136/jcp.54.5.377.
AIMS
To investigate the distribution and viral load of the most prevalent high risk human papillomavirus (HPV) types 16, 18, 31, 33, and 45 and low risk HPV types 6 and 11 in a variety of cervical lesions.
METHODS
One hundred and seventy six cytological specimens from women with different cervical lesions were investigated. For an accurate standardisation of the sample, cervical cells were counted and a volume of the cell suspension processed by polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). Semiquantitative determinations were achieved in relation to an external reference titration curve.
RESULTS
HPV DNA was detected in 60.2% of the samples. HPV-16 was the prevalent genotype (57.6%), followed by HPV-33, HPV-31, HPV-6, HPV-18, and HPV-45. HPV-11 was not detected. HPV-16 showed a pronounced increase in prevalence with the evolution of cervical disease. Semiquantitative evaluation of the results showed that only HPV-16 DNA could reach very high values (> 1000 genome copies/cell) and a very high HPV-16 load correlated with the severity of cervical disease.
CONCLUSIONS
Only HPV-16 load appears to be associated with the severity of cervical disease.
目的
研究最常见的高危人乳头瘤病毒（HPV）16、18、31、33和45型以及低危HPV 6和11型在各种宫颈病变中的分布及病毒载量。
方法
对176例患有不同宫颈病变的女性的细胞学标本进行研究。为了对样本进行准确标准化，对宫颈细胞进行计数，并取一定体积的细胞悬液通过聚合酶链反应-酶联免疫吸附测定（PCR-ELISA）进行处理。根据外部参考滴定曲线进行半定量测定。
结果
60.2%的样本中检测到HPV DNA。HPV-16是最常见的基因型（57.6%），其次是HPV-33、HPV-31、HPV-6、HPV-18和HPV-45。未检测到HPV-11。随着宫颈疾病的进展，HPV-16的流行率显著增加。结果的半定量评估显示，只有HPV-16 DNA能达到非常高的值（>1000个基因组拷贝/细胞），且非常高的HPV-16载量与宫颈疾病的严重程度相关。
结论
似乎只有HPV-16载量与宫颈疾病的严重程度相关。
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