在常规实验室环境中，筛选琼脂平板在幽门螺杆菌体外药敏试验中的作用。
Role of screening agar plates for in vitro susceptibility testing of Helicobacter pylori in a routine laboratory setting.
作者信息
Warburton-Timms V J, McNulty C A
机构信息
Public Health Laboratory, Gloucestershire Royal Hospital, Great Western Road, Gloucester GL1 3NN, UK.
出版信息
J Clin Pathol. 2001 May;54(5):408-11. doi: 10.1136/jcp.54.5.408.
BACKGROUND
Resistance of Helicobacter pylori to the more frequently used antibiotics (metronidazole and clarithromycin) reduces eradication rates even with triple treatment. Determining the antibiogram profile of H pylori can take up to 14 days and delays appropriate treatment.
AIMS
To determine the role of screening agar plates for more rapid in vitro susceptibility of H pylori to metronidazole, amoxicillin, and clarithromycin.
METHODS
Routine gastric biopsy specimens from 507 dyspeptic patients were inoculated on to 10% lysed blood agar plates containing metronidazole (8 microg/ml), clarithromycin (2 microg/ml), or amoxicillin (0.5 microg/ml). The minimum inhibitory concentration (MIC) of the 90 isolates was determined using the E test.
RESULTS
Metronidazole resistance was detected in 28 of 90 isolates by E test and nine of 98 by screening agar. The screening agar detected none of the four clarithromycin resistant isolates detected by the E test.
CONCLUSIONS
The screening agar method is not sufficiently sensitive to be used alone.
背景
幽门螺杆菌对常用抗生素（甲硝唑和克拉霉素）的耐药性会降低根除率，即使采用三联疗法也如此。确定幽门螺杆菌的药敏谱可能需要长达14天的时间，并会延误适当的治疗。
目的
确定筛选琼脂平板对幽门螺杆菌体外快速药敏试验中对甲硝唑、阿莫西林和克拉霉素的作用。
方法
将507例消化不良患者的常规胃活检标本接种到含甲硝唑（8微克/毫升）、克拉霉素（2微克/毫升）或阿莫西林（0.5微克/毫升）的10%溶血血琼脂平板上。使用E试验测定90株分离株的最低抑菌浓度（MIC）。
结果
通过E试验在90株分离株中检测到28株对甲硝唑耐药，通过筛选琼脂在98株中检测到9株。筛选琼脂未检测到E试验检测到的4株对克拉霉素耐药的分离株中的任何一株。
结论
筛选琼脂法单独使用时敏感性不足。
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