Postberg J, Juranek S A, Feiler S, Kortwig H, Jönsson F, Lipps H J
Institute for Cell Biology, University Witten/Herdecke, Stockumer Str. 10, D-58448 Witten, Germany.
J Cell Sci. 2001 May;114(Pt 10):1861-6. doi: 10.1242/jcs.114.10.1861.
Telomeric interactions with the nuclear matrix have been described in a variety of eukaryotic cells and seem to be essential for specific nuclear localization. Macronuclear DNA of hypotrichous ciliates occurs in small gene-sized DNA molecules, each being terminated by telomeres. Each macronucleus contains over 10(8 )individual DNA molecules. Owing to the high number of telomeres present in this nucleus it provides an excellent model to study telomere behaviour throughout the cell cycle. In this study we provide experimental evidence that the telomere-telomere-binding protein (TEBP) complex specifically interacts with components of the nuclear matrix in vivo. In the course of replication the specific interaction of the TEBP with components of the nuclear matrix is resolved and an attachment of the telomeres to the matrix no longer occurs.
在多种真核细胞中都已描述了端粒与核基质的相互作用，这种相互作用似乎对于特定的核定位至关重要。纤毛亚纲纤毛虫的大核DNA存在于小的基因大小的DNA分子中，每个分子都由端粒终止。每个大核包含超过10^8个单独的DNA分子。由于该核中存在大量端粒，它为研究整个细胞周期中端粒的行为提供了一个极好的模型。在本研究中，我们提供了实验证据，证明端粒-端粒结合蛋白（TEBP）复合物在体内与核基质的成分发生特异性相互作用。在复制过程中，TEBP与核基质成分的特异性相互作用消失，端粒不再附着于基质。
