Paeschke Katrin, Juranek Stefan, Rhodes Daniela, Lipps Hans Joachim
Institute of Cell Biology, University Witten/Herdecke, Stockumer Strasse 10, Witten, Germany.
Chromosome Res. 2008;16(5):721-8. doi: 10.1007/s10577-008-1222-x. Epub 2008 May 29.
It is well established that telomeres are tethered in the eukaryotic nucleus, but a detailed analysis of the regulation of telomere attachment throughout the cell cycle is still lacking. We show here that the telomeres in the macronucleus of the ciliate Stylonychia lemnae are bound to a sub-nuclear structure by an interaction of the telomere end-binding protein TEBPalpha with three SNS proteins that are integral parts of this structure. In the course of replication, the interaction of TEBPalpha with the SNS proteins is resolved and this process is regulated by cell cycle-specific phosphorylation of the SNS proteins. Our data can be incorporated into a mechanistic model for the regulation of telomere conformation and localization throughout the cell cycle.
端粒在真核细胞核中被固定，这一点已得到充分证实，但目前仍缺乏对整个细胞周期中端粒附着调控的详细分析。我们在此表明，纤毛虫类莱氏拟柱胞大核中的端粒通过端粒末端结合蛋白TEBPα与该结构的三个组成部分SNS蛋白的相互作用，与一个亚核结构相连。在复制过程中，TEBPα与SNS蛋白的相互作用被解除，这一过程受SNS蛋白的细胞周期特异性磷酸化调控。我们的数据可纳入一个关于整个细胞周期中端粒构象和定位调控的机制模型。
