使用重组EMA-1通过乳胶凝集试验检测马属动物中抗马巴贝斯虫抗体。
Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1.
作者信息
Xuan X, Igarashi I, Tanaka T, Fukumoto S, Nagasawa H, Fujisaki K, Mikami T
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
出版信息
Clin Diagn Lab Immunol. 2001 May;8(3):645-6. doi: 10.1128/CDLI.8.3.645-646.2001.
Abstract
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly between sera from B. equi-infected horses and sera from Babesia caballi-infected horses or from normal horses. The LAT results were identical to those of a previously developed enzyme-linked immunosorbent assay. These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection.
摘要
开发了一种使用重组马巴贝斯虫裂殖子抗原1（EMA-1）的乳胶凝集试验（LAT）来检测马巴贝斯虫抗体。该LAT能够非常清晰地区分感染马巴贝斯虫的马的血清与感染驽巴贝斯虫的马或正常马的血清。LAT结果与先前开发的酶联免疫吸附测定结果相同。这些结果表明，使用重组EMA-1的LAT作为诊断马巴贝斯虫感染的常规筛查方法可能非常有用。
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