[单纯疱疹病毒糖蛋白D与白细胞介素-2融合蛋白免疫对小鼠疱疹性角膜炎的影响]
[The effect of immunization with herpes simplex virus glycoprotein D fused with interluekin-2 against murine herpetic keratitis].
作者信息
Inoue T, Inoue Y, Nakamura T, Yoshida A, Inoue Y, Tano Y, Shimomura Y, Fujisawa Y, Aono A, Hayashi K
机构信息
Department of Ophthalmology, Osaka University Medical School, 2-2 Yamadaoka, Suita 565-0871, Japan.
出版信息
Nippon Ganka Gakkai Zasshi. 2001 Apr;105(4):223-9.
PURPOSE
To evaluate the effect of vaccination with fusion protein(gD-IL-2) consisting of herpes simplex type1(HSV-1) glycoprotein D(gD) and human interleukin-2(IL-2), and the effect of plasmid DNA vaccine encoding gD-IL-2 against murine herpetic keratitis.
METHODS
Plasmid containing gD-IL-2(pHDL-neol) was constructed, and gD-IL-2 peptide was purified. BALB/c mice were injected twice hypodermally or subconjunctivally with 1 microgram/0.1 ml of gD-IL-2 peptide, or twice subconjunctivally with 90 micrograms/0.05 ml of gD-IL-2 plasmid DNA. Neutralizing antibody titer and delayed-type hypersensitivity (DTH) against HSV-1 were measured. Immunized mice were challenged with CHR3 strain of HSV-1 via the cornea. The clinical picture of epithelial and stromal keratitis was scored.
RESULTS
Stromal keratitis was inhibited in gD-IL-2 peptide- or gD-IL-2 DNA-immunized mice, but epithelial keratitis was not. It was confirmed that plasmid gD-IL-2 elicited significant serum virus neutralizing titer and DTH response.
CONCLUSION
Vaccination with gD-IL-2 was effective against herpetic keratitis.
目的
评估由单纯疱疹病毒1型（HSV-1）糖蛋白D（gD）和人白细胞介素-2（IL-2）组成的融合蛋白（gD-IL-2）疫苗接种效果，以及编码gD-IL-2的质粒DNA疫苗对小鼠疱疹性角膜炎的作用。
方法
构建含gD-IL-2的质粒（pHDL-neol），纯化gD-IL-2肽。将BALB/c小鼠皮下或结膜下注射两次1微克/0.1毫升的gD-IL-2肽，或结膜下注射两次90微克/0.05毫升的gD-IL-2质粒DNA。检测针对HSV-1的中和抗体滴度和迟发型超敏反应（DTH）。经角膜用HSV-1的CHR3株攻击免疫小鼠。对上皮性和基质性角膜炎的临床表现进行评分。
结果
gD-IL-2肽或gD-IL-2 DNA免疫的小鼠基质性角膜炎受到抑制，但上皮性角膜炎未受抑制。证实质粒gD-IL-2可诱导显著的血清病毒中和滴度和DTH反应。
结论
gD-IL-2疫苗接种对疱疹性角膜炎有效。
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