Ghiasi H, Bahri S, Nesburn A B, Wechsler S L
Department of Ophthalmology Research, Cedars-Sinai Medical Center Research Institute, Los Angeles, CA 90048, USA.
Invest Ophthalmol Vis Sci. 1995 Jun;36(7):1352-60.
To compare the efficacy of each of seven expressed herpes simplex virus 1 (HSV-1) glycoproteins as vaccines to protect against ocular disease after primary ocular HSV-1 infection.
Mice were vaccinated three times with equal amounts of each of seven individually expressed HSV-1 glycoproteins (gB, gC, gD, gE, gG, gH, and gI) and then ocularly challenged with McKrae, a corneal disease-producing strain of HSV-1. Viral clearance from the eye, blepharitis, keratitis, and neovascularization were determined at various times after infection.
Mice vaccinated with gD or gB had the best protection against eye disease. Vaccination with gI, gC, or gE produced moderate protection against eye disease. Vaccination with gG produced less protection, and vaccination with gH produced no apparent protection against eye disease.
These results suggest that when used as vaccines, different HSV-1 glycoproteins provide different levels of protection against HSV-1-induced eye disease. Based on comparison with the authors' previously published results, the ability of each glycoprotein to protect against eye disease correlated with the ability of the glycoprotein to induce high serum neutralizing antibody titers and killer cell activity. Results suggest that the effectiveness of these seven glycoproteins in protecting against eye disease can be ranked as follows: gD > gB > gI > (gC = gE) > gG > gH.
比较七种表达的单纯疱疹病毒1型（HSV-1）糖蛋白作为疫苗在原发性眼部HSV-1感染后预防眼部疾病的效果。
用等量的七种单独表达的HSV-1糖蛋白（gB、gC、gD、gE、gG、gH和gI）对小鼠进行三次免疫接种，然后用HSV-1的角膜致病株McKrae进行眼部攻击。在感染后的不同时间测定眼部的病毒清除情况、睑缘炎、角膜炎和新生血管形成情况。
用gD或gB免疫接种的小鼠对眼部疾病的保护效果最佳。用gI、gC或gE免疫接种对眼部疾病产生中等程度的保护作用。用gG免疫接种产生的保护作用较小，而用gH免疫接种对眼部疾病没有明显的保护作用。
这些结果表明，当用作疫苗时，不同的HSV-1糖蛋白对HSV-1诱导的眼部疾病提供不同程度的保护。与作者先前发表的结果相比，每种糖蛋白预防眼部疾病的能力与该糖蛋白诱导高血清中和抗体滴度和杀伤细胞活性的能力相关。结果表明，这七种糖蛋白在预防眼部疾病方面的有效性可按以下顺序排列：gD > gB > gI > （gC = gE） > gG > gH。
