Kr zel A, Lesniak W, Jezowska-Bojczuk M, Mlynarz P, Brasuñ J, Kozlowski H, Bal W
Faculty of Chemistry, University of Wrocław, Poland.
J Inorg Biochem. 2001 Mar;84(1-2):77-88. doi: 10.1016/s0162-0134(00)00212-9.
D,L-Dithiothreitol (DTT), known also as Cleland reagent, is a thiol group protectant, used commonly in peptide and protein chemistry. Therefore, it is often added at high concentrations in preparations of proteins relevant to heavy metal biochemistry. The coordination of five of these metal ions, Zn(II), Cd(II), Pb(II), Ni(II) and Cu(I) to DTT was studied by means of potentiometric titrations, and UV-Vis and NMR spectroscopies. It was found that DTT forms specific and very stable polymeric and monomeric complexes with all of these metal ions, using both of its sulfur donors. The quantitative description of these complexes in solution and the solid state provides the basis for predictions of interference from DTT in studies of metal ion binding of thiol-containing biomolecules.
D，L-二硫苏糖醇（DTT），也被称为克莱兰试剂，是一种硫醇基团保护剂，常用于肽和蛋白质化学领域。因此，在与重金属生物化学相关的蛋白质制剂中，它常常以高浓度添加。通过电位滴定法、紫外可见光谱法和核磁共振光谱法研究了其中五种金属离子，即锌（II）、镉（II）、铅（II）、镍（II）和铜（I）与DTT的配位情况。研究发现，DTT利用其两个硫供体与所有这些金属离子形成特定且非常稳定的聚合物和单体配合物。对这些配合物在溶液和固态中的定量描述为预测DTT在含硫醇生物分子的金属离子结合研究中的干扰提供了依据。
