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从商业化养殖的拟线形海囊藻(Itomozuku)中分离和鉴定海藻酸

Isolation and characterizartion of alginic acid from commercially cultured Nemacystus decipiens (Itomozuku).

作者信息

Tako M, Kiyuna S, Uechi S, Hongo F

机构信息

Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan.

出版信息

Biosci Biotechnol Biochem. 2001 Mar;65(3):654-7. doi: 10.1271/bbb.65.654.

DOI:10.1271/bbb.65.654
PMID:11330683
Abstract

An alginate was isolated from commercially cultured Nemacystus decipiens which had been harvested in Yonashiro Town (Okinawa, Japan). The yield of the alginate was 1.6% (w/w of wet alga), and the uronic acid, ash and moisture contents of the alginate were 86.0%, 12.0%, and 2.3% (w/w), respectively. The molecular mass of the alginate was estimated to be about 1.5 x 10(5). The infrared spectrum and optical rotation of the alginate were in agreement with those of the standard alginate. D-Mannuronic acid and L-guluronic acid were identified by 1H- and 13C-NMR spectroscopy, the molar ratio of both sugar residues being estimated to be 0.72:1.00.

摘要

从日本冲绳县与那城商业养殖并收获的错综皮丝藻中分离出一种藻酸盐。藻酸盐的产量为1.6%(占湿藻重量的百分比),该藻酸盐的糖醛酸、灰分和水分含量分别为86.0%、12.0%和2.3%(均为重量百分比)。估计该藻酸盐的分子量约为1.5×10⁵。该藻酸盐的红外光谱和旋光度与标准藻酸盐一致。通过¹H-和¹³C-核磁共振光谱鉴定出D-甘露糖醛酸和L-古洛糖醛酸,两种糖残基的摩尔比估计为0.72:1.00。

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