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Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting.

作者信息

Takai T, Akagawa-Chihara M, Yokota T, Okumura Y

机构信息

Bioscience Research and Development Laboratory, Asahi Breweries, Ltd., Kitasoma-gun, Ibaraki, Japan.

出版信息

Biosci Biotechnol Biochem. 2001 Mar;65(3):694-7. doi: 10.1271/bbb.65.694.

DOI:10.1271/bbb.65.694
PMID:11330693
Abstract

A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis.

摘要

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