Decker C H, Visser J, Schreier P
Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Germany.
Appl Microbiol Biotechnol. 2001 Mar;55(2):157-63. doi: 10.1007/s002530000462.
From Aspergillus tubingensis CBS 643.92 four distinct beta-glucosidases (I-IV) were purified by a four-step purification procedure. SDS-PAGE revealed molecular masses of 131, 126, 54 and 54 kDa, respectively, and their isoelectric points were determined to be 4.2, 3.9, 3.7 and 3.6, respectively. The beta-glucosidases exhibited high diversity with respect to pH and temperature optima and stability, as well as to substrate specificity and glucose tolerance. The major beta-glucosidase (I) preferentially hydrolysed oligosaccharides. The acid-stable and heat-tolerant beta-glucosidase II hydrolysed aryl and terpenyl beta-D-glucosides as well as 1-O-trans-cinnamoyl beta-D-glucoside. In contrast to beta-glucosidases I and II, the minor beta-glucosidases III and IV were found to be glucose-tolerant; inhibition constants of 470 and 600 mM, respectively, were determined.
通过四步纯化程序从烟曲霉CBS 643.92中纯化出四种不同的β-葡萄糖苷酶（I-IV）。SDS-PAGE显示其分子量分别为131、126、54和54 kDa，其等电点分别测定为4.2、3.9、3.7和3.6。这些β-葡萄糖苷酶在最适pH和温度以及稳定性、底物特异性和葡萄糖耐受性方面表现出高度多样性。主要的β-葡萄糖苷酶（I）优先水解寡糖。耐酸且耐热的β-葡萄糖苷酶II水解芳基和萜基β-D-葡萄糖苷以及1-O-反式肉桂酰基β-D-葡萄糖苷。与β-葡萄糖苷酶I和II不同，发现次要的β-葡萄糖苷酶III和IV具有葡萄糖耐受性；分别测定其抑制常数为470和600 mM。
